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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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STAT5A Antibody (C‑Terminus) LS‑C10412
STAT5A antibody LS-C10412 is an unconjugated mouse monoclonal antibody to STAT5A from human, mouse and rat. Validated for ELISA, GS, IP and WB.
Antibody
Catalog
Size
Price
LS-C10412-100
100 µg
$605

Popular STAT5A Products

Immunohistochemistry of paraffin-embedded human breast carcinoma tissue using STAT5A(Ab-780) antibody (left) or the same antibody preincubated with blocking peptide (right).
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Human
Applications: Flow Cytometry
Anti-STAT5A antibody IHC staining of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human, Mouse, Rat, Bovine, Pig
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation
Anti-STAT5A antibody IHC staining of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Anti-STAT5A antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10045 concentration 10 ug/ml.
Species: Human, Monkey, Rat, Dog
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry

Product Description

STAT5A antibody LS-C10412 is an unconjugated mouse monoclonal antibody to STAT5A from human, mouse and rat. Validated for ELISA, GS, IP and WB.
About STAT5A
STAT5A is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. P42229 NM_003152 NP_003143.2

STAT5A Antibody, MGF Antibody, STAT5 Antibody


Specifications

Target
Mouse STAT5A
Host
Mouse
Reactivity
Mouse, Human, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1 - 3 µg/ml)
  • Immunoprecipitation
  • ELISA (0.1 - 1 µg/ml)
  • Gel shift
  • (applications tested for the base form of this product only)
Immunogen
STAT5A antibody was raised against synthetic peptide corresponding to the C-terminus of murine Stat5a (carrier protein coupled).
Epitope
C-Terminus
Specificity
Reacts specifically with the STAT5a protein. Does not cross-react with the highly related STAT5b protein. Cross-reactivity with other endogenous STAT isoforms has not been observed. Reactivity has been confirmed by Western Blot using cell lysates derived from human A431 cells (+/-EGF treatment), NIH 3T3 cells, MCF-7 cells and HeLa cells. Species cross-reactivity: Human, mouse and rat.
Usage
Suitable for use in ELISA, Western Blot, Immunoprecipitation and Gel Mobility Shift Assay. ELISA: 0.1-1 ug/ml. Immunoprecipitation: 5 ug. Western Blot: 1-3 ug/ml. Gel Mobility Shift Assay: 1-3ug.
Presentation
PBS, pH 7.4, 0.1% sodium azide.
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: United States
Date Requested: 7/20/2018

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