Mouse, Human, Rat
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
- Western blot (1 - 3 µg/ml)
- ELISA (0.1 - 1 µg/ml)
- Gel shift
- (applications tested for the base form of this product only)
STAT5A antibody was raised against synthetic peptide corresponding to the C-terminus of murine Stat5a (carrier protein coupled).
Reacts specifically with the STAT5a protein. Does not cross-react with the highly related STAT5b protein. Cross-reactivity with other endogenous STAT isoforms has not been observed. Reactivity has been confirmed by Western Blot using cell lysates derived from human A431 cells (+/-EGF treatment), NIH 3T3 cells, MCF-7 cells and HeLa cells. Species cross-reactivity: Human, mouse and rat.
Suitable for use in ELISA, Western Blot, Immunoprecipitation and Gel Mobility Shift Assay. ELISA: 0.1-1 ug/ml. Immunoprecipitation: 5 ug. Western Blot: 1-3 ug/ml. Gel Mobility Shift Assay: 1-3ug.
PBS, pH 7.4, 0.1% Sodium Azide
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.