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Anti-STAT5A Antibody (C-Terminus) LS-C10412

Ordering

Wt. Vol. Conc. Price
100 µg - - $495
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
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Mouse Monoclonal (IgG1,k) to Mouse STAT5A
Mouse, Human, Rat
Western blot, Immunoprecipitation, ELISA, Gel shift
Unconjugated

Details

Mouse STAT5A
Mouse
Mouse, Human, Rat (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal
Immunoaffinity purified

Applications

Western blot (1 - 3 µg/ml), Immunoprecipitation, ELISA (0.1 - 1 µg/ml), Gel shift

Specificity and Use

STAT5A antibody was raised against synthetic peptide corresponding to the C-terminus of murine Stat5a (carrier protein coupled).
C-Terminus
Reacts specifically with the STAT5a protein. Does not cross-react with the highly related STAT5b protein. Cross-reactivity with other endogenous STAT isoforms has not been observed. Reactivity has been confirmed by Western Blot using cell lysates derived from human A431 cells (+/-EGF treatment), NIH 3T3 cells, MCF-7 cells and HeLa cells. Species cross-reactivity: Human, mouse and rat.
Suitable for use in ELISA, Western Blot, Immunoprecipitation and Gel Mobility Shift Assay. ELISA: 0.1-1 ug/ml. Immunoprecipitation: 5 ug. Western Blot: 1-3 ug/ml. Gel Mobility Shift Assay: 1-3ug.

Packaging

PBS, pH 7.4, 0.1% sodium azide.
Long term: Add glycerol (40-50%) -20°C; Short term: +4°C
For research use only.

About STAT5A

P42229 NM_003152 NP_003143.2

STAT5A Antibody, MGF Antibody, STAT5 Antibody

STAT5A is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones.

Requested From: United States
Date Requested: 9/27/2016

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