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Anti-STAT5A Antibody (aa774-793) LS-C10407

Ordering

Wt. Vol. Conc. Price
- 100 µl - $530
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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Rabbit Polyclonal (IgG) to Mouse STAT5A
Mouse
Western blot, Immunoprecipitation
Unconjugated

Details

Mouse STAT5A
Rabbit
Mouse (tested or 100% immunogen sequence identity)
Human, Monkey, Rat, Horse (at least 90% immunogen sequence identity)
IgG Polyclonal
Antiserum

Applications

Western blot, Immunoprecipitation

Specificity and Use

STAT5A antibody was raised against kLH-conjugated, synthetic peptide (LDARLSPPAGLFTSARSSLS) corresponding to amino acids 774-793 of mouse STAT5A. Percent identity by BLAST analysis: Mouse (100%); Rat (95%); Human, Gorilla, Monkey, Horse (90%); Hamster, Panda, Dog (85%); Rabbit, Pig (80%).
aa774-793
Recognizes and specific for mouse STAT5A at Mr ~94kD. Species cross-reactivity: rat and human.
Western Blot Analysis: A 1:2000 dilution detected STAT5A in RIPA lysates from Nb2-SP cells. Immunoprecipitation: 4 ul immunoprecipitated STAT5A from 500 ug of Nb2-SP RIPA lysate. Western Blot Analysis: Nb2-SP cell lysate (20 ug) was resolved by electrophoresis, transferred to nitro-cellulose and probed with anti-STAT5A (1:2000). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation/Western Blot Analysis: STAT5A was immunoprecipitated from Nb2-SP cell lysate (500 ug) using anti-STAT5A (4ul). The reduced immuno-complex was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-STAT5A (1:2000), lane 2, or the secondary antibody alone, lane 1. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.

Packaging

Rabbit antiserum
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About STAT5A

P42229 NM_003152 NP_003143.2

STAT5A Antibody, MGF Antibody, STAT5 Antibody

STAT5A is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones.

Requested From: United States
Date Requested: 9/28/2016

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