Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human, Mouse, Rat, Bovine (tested or 100% immunogen sequence identity)
Western blot (1:500 - 1:1000)
Specificity and Use
STAT5 A+B antibody was raised against kLH-conjugated, synthetic peptide containing the sequence. DGpYVK. in which pY corresponds to phospho-tyrosine 694 of human STAT5.
Recognizes phospho-STAT5A/B (Tyr694/699), Mr 95kD. An unknown protein was also detected, Mr 200kD. Species cross-reactivity: Human, bovine, rat and mouse.
Suitable for use in Western Blot, Peptide Inhibition and Peptide Dot Blot. Western Blot: 1:500-1:1000 detects phospho-STAT5A/B (Tyr694/699) in lysates from 3T3/NIH cells treated with PDGF. Non-treated and PDGF treated 3T3/NIH cells were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho-STAT5A/B (Tyr694/699) (1:500 dilution). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Peptide Inhibition: 1 uM of the peptide containing the phospho-STAT5A/B (Tyr694/699) modification completely abolished detection of STAT5 by anti-phospho-STAT5A/B (Tyr694/699) in immunoblot analysis of 3T3/NIH cell lysates ( lane 2). 3T3/NIH cell lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho-STAT5A/B (Tyr694/699) (1:500 dilution) or anti-phospho- STAT5A/B (Tyr694/699) pre-absorbed with 1 uM peptides. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Peptide Dot Blot: 1:500-1:2000 detected the peptide containing phospho-STAT5A/B (Tyr694/699). A dilution series of peptides. 2 ul of each dilution was spotted onto PVDF membranes and probed with anti-phospho-STAT5A/B (Tyr694/699) (1:1000 dilution). Peptides were visualized using a goat anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05% sodium azide, 30% glycerol.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.