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Anti-STAT5 A+B Antibody (phospho-Tyr694/Tyr699) LS-C10420


Wt. Vol. Conc. Price
- 100 µl - $530
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

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Most Popular STAT5 A+B Antibodies

Anti-STAT5 A+B Antibody (phospho-Tyr694) LS-C39269
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IHC - Paraffin, Western blot, Immunoprecipitation, Flow Cytometry
Anti-STAT5 A+B Antibody (C-Terminus) LS-C51708
Rabbit Polyclonal to Mouse STAT5 A+B
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Anti-STAT5 A+B Antibody (phospho-Tyr694/Tyr699) LS-C354261
Rabbit Polyclonal to Human STAT5 A+B
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IHC - Paraffin, Western blot
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human STAT5 A+B
Human, Mouse, Rat, Bovine
Western blot, Functional Assay


Human STAT5 A+B
Human, Mouse, Rat, Bovine (tested or 100% immunogen sequence identity)
IgG Polyclonal
Immunoaffinity purified


  • Western blot (1:500 - 1:1000)
  • Functional Assay

Specificity and Use

STAT5 A+B antibody was raised against kLH-conjugated, synthetic peptide containing the sequence. DGpYVK. in which pY corresponds to phospho-tyrosine 694 of human STAT5.
Recognizes phospho-STAT5A/B (Tyr694/699), Mr 95kD. An unknown protein was also detected, Mr 200kD. Species cross-reactivity: Human, bovine, rat and mouse.
Suitable for use in Western Blot, Peptide Inhibition and Peptide Dot Blot. Western Blot: 1:500-1:1000 detects phospho-STAT5A/B (Tyr694/699) in lysates from 3T3/NIH cells treated with PDGF. Non-treated and PDGF treated 3T3/NIH cells were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho-STAT5A/B (Tyr694/699) (1:500 dilution). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Peptide Inhibition: 1 uM of the peptide containing the phospho-STAT5A/B (Tyr694/699) modification completely abolished detection of STAT5 by anti-phospho-STAT5A/B (Tyr694/699) in immunoblot analysis of 3T3/NIH cell lysates ( lane 2). 3T3/NIH cell lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho-STAT5A/B (Tyr694/699) (1:500 dilution) or anti-phospho- STAT5A/B (Tyr694/699) pre-absorbed with 1 uM peptides. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Peptide Dot Blot: 1:500-1:2000 detected the peptide containing phospho-STAT5A/B (Tyr694/699). A dilution series of peptides. 2 ul of each dilution was spotted onto PVDF membranes and probed with anti-phospho-STAT5A/B (Tyr694/699) (1:1000 dilution). Peptides were visualized using a goat anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.


0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05% sodium azide, 30% glycerol.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About STAT5 A+B

Requested From: United States
Date Requested: 10/25/2016

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