STAT5 A+B antibody LS-C10419 is an unconjugated mouse monoclonal antibody to STAT5 A+B from human, mouse, rat and other species. Validated for WB.
Human, Mouse, Rat, Bovine, Sheep
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
Protein G purified
- Western blot
- (applications tested for the base form of this product only)
STAT5 A+B antibody was raised against synthetic phospho-peptide (KAVDG[pY]VKPQIK) corresponding to amino acids 689-700 of STAT5A and 694-705 of STAT5B.
Specific for the phosphorylated tyrosine residues 694 of STAT5A (94kD) and 699 of STAT5B (92kD).
0.5-1 ug/ml. Western Blot Analysis: 0.5-2 mg/ml detects phosphorylated STAT5A/B in lysates from 3T3 cells stimulated with PDGF and from HeLa cells stimulated with interferon-a (IFN-a). HeLa cell lysates were prepared from cells stimulated with (lane 1) and without (lane 2) IFN-a. The lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-pSTAT5A/ B (0.5 mg/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
0.1 M Tris-Glycine, 0.15 M NaCl, 0.05% Sodium Azide
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.