Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human, Mouse, Rat, Bovine, Sheep (tested or 100% immunogen sequence identity)
Protein G purified
Specificity and Use
STAT5 A+B antibody was raised against synthetic phospho-peptide (KAVDG[pY]VKPQIK) corresponding to amino acids 689-700 of STAT5A and 694-705 of STAT5B.
Specific for the phosphorylated tyrosine residues 694 of STAT5A (94kD) and 699 of STAT5B (92kD).
0.5-1 ug/ml. Western Blot Analysis: 0.5-2 mg/ml detects phosphorylated STAT5A/B in lysates from 3T3 cells stimulated with PDGF and from HeLa cells stimulated with interferon-a (IFN-a). HeLa cell lysates were prepared from cells stimulated with (lane 1) and without (lane 2) IFN-a. The lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-pSTAT5A/ B (0.5 mg/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
0.1 M Tris-glycine, 0.15 M sodium chloride, 0.05% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.