Human STAT5 A+B

Mouse

Human, Mouse, Rat, Bovine, Sheep (tested or 100% immunogen sequence identity)

IgG Monoclonal

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Protein G purified

Unmodified

Synthetic phospho-peptide (KAVDG[pY]VKPQIK) corresponding to amino acids 689-700 of STAT5A and 694-705 of STAT5B.

pTyr694/Tyr699

Specific for the phosphorylated tyrosine residues 694 of STAT5A (94kD) and 699 of STAT5B (92kD).

• Western blot

0.5-1 ug/ml. Western Blot Analysis: 0.5-2 mg/ml detects phosphorylated STAT5A/B in lysates from 3T3 cells stimulated with PDGF and from HeLa cells stimulated with interferon-a (IFN-a). HeLa cell lysates were prepared from cells stimulated with (lane 1) and without (lane 2) IFN-a. The lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-pSTAT5A/ B (0.5 mg/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.

0.1 M Tris-Glycine, 0.15 M NaCl, 0.05% Sodium Azide

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.