Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
IHC - Paraffin, Western blot, Immunoprecipitation, Flow Cytometry
Rat STAT5 A+B
Rat, Human, Mouse (tested or 100% immunogen sequence identity)
Bovine (at least 90% immunogen sequence identity)
Tissue culture supernatant
IHC - Paraffin
Western blot (1:1000)
Flow Cytometry (1:100)
Specificity and Use
STAT5 A+B antibody was raised against synthetic peptide (KLH-coupled) corresponding to residues surrounding Tyr694 of Stat5. Species Sequence Homology: Bovine, monkey, rat - 100%.
Detects endogenous levels of Stat5 protein when phosphorylated at Tyr694. Species cross-reactivity: Human and mouse.
Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation and Immunohistochemistry. Western Blot: 1:1000. Immunoprecipitation: 1:50. Immunohistochemistry (paraffin): 1:300. Antigen retrieval with citrate buffer is recommended. Flow Cytometry: 1:100.
10 mM sodium HEPES, pH 7.5, 150 mM sodium chloride, 100 ug/ml BSA, 50% glycerol, less than 0.02% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.