Reactivity
Mouse, Human, Rat
(tested or 100% immunogen sequence identity)
Predicted
Human, Rat (at least 90% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Affinity purified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues near the carboxy terminus of mouse Stat5a protein.
Specificity
This antibody is specific for mouse Stat5 protein. A BLAST analysis was used to suggest cross-reactivity with Stat5 from human, mouse and rat based on 100% homology with the immunizing sequence. Cross-reactivity with Stat5 from other sources has not been determined.
Applications
- Western blot (1:500 - 1:2000)
- ELISA (1:25000)
Usage
This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 91 kD in size corresponding to phosphorylated Stat5 protein by western blotting in the appropriate cell lysate or extract.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: -20°C; Long term: -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee