Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse, Human, Rat (tested or 100% immunogen sequence identity)
Western blot (1:500 - 1:2000)
Specificity and Use
STAT5 A+B antibody was raised against synthetic peptide corresponding to residues near the carboxy terminus of mouse Stat5 alpha protein.
A BLAST analysis was used to suggest cross-reactivity with Stat5 from human, mouse and rat based on 100% homology with the immunizing sequence. Cross-reactivity with Stat5 from other sources has not been determined
This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 91 kD in size corresponding to phosphorylated Stat5 protein by western blotting in the appropriate cell lysate or extract.
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Anti-STAT5 Antibody - Western Blot. Western blot of affinity purified anti-STAT5 antibody shows detection of Stat5 protein in HeLa cell extract (arrowhead). Primary antibody was used at a 1:600 dilution reacted overnight at 4C. Secondary antibody was used at 1:20000. Molecular weight estimation was made by comparison to prestained MW markers. Identity of the upper and lower bands is unknown. Personal communication, Luanne Lukes, NCI, Bethesda, MD.