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Anti-STAT2 Antibody (C-Terminus) IHC-plus™ LS-B1649

Note: This antibody replaces LS-C40914
Catalog Size Price
LS-B1649-50 50 µl Unavailable

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100% Guaranteed
Rabbit Polyclonal (IgG) to Human STAT2
Human
IHC - Paraffin, Western blot, Immunoprecipitation
Unconjugated

Details

Human STAT2
Rabbit
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (1:50)
  • Western blot
  • Immunoprecipitation

Specificity and Use

STAT2 antibody was raised against synthetic peptide from human STAT2.
C-Terminus
Peptide mapping to the carboxy terminus of human STAT2
Immunohistochemistry: LS-B1649 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1649 was determined to be 1:50.

Packaging

Phosphate-buffered solution, pH 7.2, 0.09% sodium azide, 0.2% gelatin.
+4°C, avoid freezing.
For research use only.

About STAT2

P52630 NM_005419 NP_005410.1

STAT2 Antibody, ISGF-3 Antibody, STAT113 Antibody, p113 Antibody

STAT2 is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators.

Immunohistochemistry

Immunohistochemistry
Anti-STAT2 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1649 dilution 1:50.

Western blot

Western blot
Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-STAT2 polyclonal antibody. Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence system.

Requested From: 
Date Requested: 10/20/2017

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