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Anti-STAT1 Antibody (phospho-Tyr701) LS-C10368

Catalog Size Price
LS-C10368-100 100 µl Unavailable

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189 STAT1 Antibodies

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Anti-STAT1 Antibody (phospho-Tyr701) LS-C10368
Rabbit Polyclonal (IgG) to Mouse STAT1
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Anti-STAT1 Antibody (clone SM1) IHC-plus™ LS-B1874
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Anti-STAT1 Antibody (N-Terminus) IHC-plus™ LS-B4583
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Anti-STAT1 Antibody (N-Terminus, Biotin) LS-C437762
Rabbit Polyclonal (IgG) to Human STAT1
Human, Chimpanzee, Orangutan, Gibbon, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Sheep, Chicken, Zebrafish
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100% Guaranteed
Rabbit Polyclonal (IgG) to Mouse STAT1
Mouse
Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Mouse STAT1
Rabbit
IgG Polyclonal
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Immunoaffinity purified
Unmodified

Applications

  • Western blot (1:500 - 1:2500)
  • (applications tested for the base form of this product only)

  • IHC - Paraffin

Specificity and Use

STAT1 antibody was raised against kLH-conjugated synthetic peptide (C-DDPKRTG[pY]IKTE) corresponding to amino acids 694-705 of mouse STAT1, containing a N-terminal cysteine.
pTyr701
Recognizes phosphorylated STAT1, Mr 92kD. Species cross-reactivity: Human, mouse, rat and chicken.
Suitable for use in Western Blot. Western Blot: 1:500-1:2500. A 1:500-1:2500 dilution detects phosphorylated STAT1 (92kD) in RIPA lysates from CNTF-treated SK-N-MC cells. 20 ug of non-treated or CNTF-treated SK-N-MC cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho-STAT1 (Y107) (1:1000). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Western Blot Protocol. 1. Perform SDS-polyacrylamide gel electrophoresis (SDS-PAGE) on an appropriately treated cell lysate sample (cell lysis buffer: 50 mM Tris-HCl, pH7.4; 1% NP-40; 0.25% sodium deoxycholate; 150 mM NaCl; 1 mM EGTA; 1 mM PMSF; 1 ug/ml each aprotinin, leupeptin, pepstatin; 1 mM Na3VO4; 1 mM NaF) and transfer the proteins to nitrocellulose. Wash the blotted nitrocellulose twice with water. 2. Block the blotted nitrocellulose in freshly prepared TBS containing 5% nonfat dry milk and 0.05% Tween-20 (TBST-MLK) for 30-60 minutes at room temperature with constant agitation. 3. Incubate the nitrocellulose with a 1:500-1:2500 dilution of anti-phospho-STAT1 (Y701), diluted in freshly prepared TBST-MLK overnight with agitation at 4?C. 4. Wash the nitrocellulose twice with water. 5. Incubate the nitrocellulose in the secondary reagent of choice (a goat anti-rabbit HRP conjugated IgG, 1:5000 to dilution was used) in TBST-MLK for 1.5 hours at room temperature with agitation. 6. Wash the nitrocellulose with water twice. 7. Wash the nitrocellulose in TBS-0.05% Tween 20 for 3-5 minutes. 8. Rinse the nitrocellulose in 4-5 changes of water. 9. Use detection method of choice (enhanced chemiluminescence was used).

Packaging

PBS, 5 mg/ml BSA, 0.1% sodium azide, 50% glycerol
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About STAT1

P42224 NM_007315 NP_009330.1

STAT1 Antibody, ISGF-3 Antibody, STAT91 Antibody, CANDF7 Antibody

Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors. Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2.

Requested From: 
Date Requested: 7/25/2017

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