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Anti-STAT1 Antibody (phospho-Tyr701) LS-C59556

Ordering

Wt. Vol. Conc. Price
100 µg - 2.3 mg/ml $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular STAT1 Antibodies

Anti-STAT1 Antibody (clone SM1) IHC-plus™ LS-B1874
Mouse Monoclonal [clone SM1] (IgG2b) to Human STAT1
Human, Monkey, Mouse, Bat, Bovine, Dog, Hamster, Horse, Pig, Sheep
IHC - Paraffin, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry Image
Anti-STAT1 Antibody (N-Terminus) IHC-plus™ LS-B4583
Rabbit Polyclonal (IgG) to Human STAT1
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Sheep, Chicken, Zebrafish
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Mouse STAT1
Mouse, Rat
Western blot, ELISA
Unconjugated

Details

Mouse STAT1
Rabbit
Mouse, Rat (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Protein A purified
Unmodified

Applications

  • Western blot (1:500 - 1:2000)
  • ELISA (1:25000)

Specificity and Use

STAT1 antibody was raised against synthetic peptide corresponding to residues surrounding Y701 in the mouse STAT1 protein.
pTyr701
A BLAST analysis was used to suggest cross-reactivity with Stat1 from mouse and rat based on 100% homology with the immunizing sequence. Partial reactivity is expected against Stat1 from human sources as 90% homology is noted. Cross-reactivity with Stat1 from other sources has not been determined
This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 91 kD in size corresponding to phosphorylated Stat1 protein by western blotting in the appropriate cell lysate or extract. This phospho-specific polyclonal antibody reacts with mouse Stat1 pY701 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About STAT1

P42224 NM_007315 NP_009330.1

STAT1 Antibody, ISGF-3 Antibody, STAT91 Antibody, CANDF7 Antibody

Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors. Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2.

Western blot

Western blot
Anti-Stat1 pY701 Antibody - Western Blot. Western blot of Protein A purified anti-Stat1 pY701 antibody shows detection of phosphorylated Stat1 (indicated by arrowhead at ~91 kD) in K562 cells after 30 min treatment with 1Ku of hIFN-??(lane 2). No reactivity is seen for non-phosphorylated Stat1 in untreated cells (lane 1). The membrane was probed with the primary antibody at a 1:1000 dilution at 4C, overnight. Personal Communication from Ana Gamero, CCR-NCI, Bethesda, MD.
 

Requested From: United States
Date Requested: 12/10/2016

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