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Anti-STAT1 Antibody (C-Terminus) LS-C10355

Ordering

Wt. Vol. Conc. Price
250 µg - - $530
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
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Rabbit Polyclonal (IgG) to Human STAT1
Human, Mouse, Rat
Western blot, Immunoprecipitation
Unconjugated

Details

Human STAT1
Rabbit
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
IgG Polyclonal
Protein A purified

Applications

Western blot (0.5 - 2 µg/ml), Immunoprecipitation

Specificity and Use

STAT1 antibody was raised against gST fusion-protein containing the carboxy terminal 36 amino acids of human STAT1a. This sequence is missing in the b isoform.
C-Terminus
Recognizes the STAT1 protein, Mr 91kD. of human, mouse and rat. Other species cross-reactivity is unknown.
Suitable for use in Western Blot, Immunoprecipitation. Western Blot: 0.5-2 ug/ml detects STAT1 in RIPA lysates from human Jurkat, mouse CTLL and rat L6 cells. Jurkat cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-STAT1 (1 ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 4 mg immunoprecipitated STAT1 from 500 mg of human Jurkat RIPA lysate.

Packaging

250 ul of 0.07 M Tris-glycine, 0.105 M sodium chloride, 0.035% sodium azide, 30% glycerol.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About STAT1

P42224 NM_007315 NP_009330.1

STAT1 Antibody, ISGF-3 Antibody, STAT91 Antibody, CANDF7 Antibody

Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors. Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2.

Requested From: United States
Date Requested: 9/25/2016

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