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Anti-STAT1 Antibody (C-Terminus) IHC-plus™ LS-B591

Note: This antibody replaces LS-C252, LS-C124584, LS-C10361

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular STAT1 Antibodies

Anti-STAT1 Antibody (clone SM1) IHC-plus™ LS-B1874
Mouse Monoclonal [clone SM1] (IgG2b) to Human STAT1
Human, Monkey, Mouse, Bat, Bovine, Dog, Hamster, Horse, Pig, Sheep
IHC - Paraffin, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry Image
Anti-STAT1 Antibody (N-Terminus) IHC-plus™ LS-B4583
Rabbit Polyclonal (IgG) to Human STAT1
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Sheep, Chicken, Zebrafish
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human STAT1
Human, Mouse, Rat
IHC - Paraffin, ICC, Western blot, Immunoprecipitation
Unconjugated

Details

Human STAT1
Rabbit
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Ion exchange chromatography
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • ICC
  • Western blot (1:1000 - 1:2000)
  • Immunoprecipitation

Specificity and Use

STAT1 antibody was raised against synthetic peptide from human STAT1.
LS-E29833 - Liquid - 50 µg - $145.00
C-Terminus
peptide corresponding to amino acids near the carboxy terminus of human STAT1a The sequences differ from the murine corresponding sequences by four amino acids
Immunohistochemistry: LS-B591 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B591 was determined to be 5 ug/ml.

Packaging

PBS, 0.02% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
For research use only.

About STAT1

P42224 NM_007315 NP_009330.1

STAT1 Antibody, ISGF-3 Antibody, STAT91 Antibody, CANDF7 Antibody

Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors. Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2.

Immunohistochemistry

Immunohistochemistry
Anti-STAT1 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B591 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-STAT1 antibody IHC of human lung, alveolar macrophages. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B591 concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry
Immunocytochemistry of STAT1 alpha in HeLa cells with STAT1 alpha antibody at 10 ug/ml.

Western blot

Western blot
Western blot of STAT1 alpha in whole cell lysates from HeLa (H), Jurkat (J), A431 (A), K562 (K), and NIH3T3 (N) cells, with STAT1a antibody at 1 ug/ml.

Requested From: United States
Date Requested: 12/7/2016

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