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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ST8SIA4 Antibody (aa186‑214) LS‑C161534
ST8SIA4 antibody LS-C161534 is an unconjugated rabbit polyclonal antibody to ST8SIA4 from human and mouse. Validated for Flow, IHC and WB.
Catalog
Size
Price
LS-C161534-400
400 µl
Unavailable

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Product Description

ST8SIA4 antibody LS-C161534 is an unconjugated rabbit polyclonal antibody to ST8SIA4 from human and mouse. Validated for Flow, IHC and WB.
About ST8SIA4
ST8SIA4 catalyzes the polycondensation of alpha-2,8-linked sialic acid required for the synthesis of polysialic acid, a modulator of the adhesive properties of neural cell adhesion molecule (NCAM1). The encoded protein, which is a member of glycosyltransferase family 29, is a type II membrane protein that may be present in the Golgi apparatus. Two transcript variants encoding different isoforms have been found for this gene. Q92187 NM_005668 NP_005659.1

ST8SIA4 Antibody, Alpha-2,8-sialyltransferase 8D Antibody, PST Antibody, Sialyltransferase 8D Antibody, SIAT8D Antibody, ST8SiaIV Antibody, Polysialyltransferase-1 Antibody, SIAT8-D Antibody, Sialytransferase St8Sia IV Antibody, ST8SIA-IV Antibody


Specifications

Target
Human ST8SIA4
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Predicted
Cow (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:10 - 1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E10307 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C161534. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa186-214
Specificity
This ST8SIA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 186-214 amino acids from the Central region of human ST8SIA4.
Presentation
PBS, 0.09% sodium azide
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

ST8SIA4 Antibody IHC of formalin-fixed and paraffin-embedded brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
ST8SIA4 Antibody IHC of formalin-fixed and paraffin-embedded brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

Western blot of ST8SIA4 Antibody in HL-60 cell line lysates (35 ug/lane). ST8SIA4 (arrow) was detected using the purified antibody. Western blot of ST8SIA4 Antibody in mouse spleen tissue lysates (35 ug/lane). ST8SIA4 (arrow) was detected using the purified antibody.
Western blot of ST8SIA4 Antibody in HL-60 cell line lysates (35 ug/lane). ST8SIA4 (arrow) was detected using the purified antibody. Western blot of ST8SIA4 Antibody in mouse spleen tissue lysates (35 ug/lane). ST8SIA4 (arrow) was detected using the purified antibody.

Flow Cytometry

ST8SIA4 Antibody flow cytometry of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
ST8SIA4 Antibody flow cytometry of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

ST8SIA4 Antibody IHC of formalin-fixed and paraffin-embedded brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
ST8SIA4 Antibody IHC of formalin-fixed and paraffin-embedded brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

Western blot of ST8SIA4 Antibody in HL-60 cell line lysates (35 ug/lane). ST8SIA4 (arrow) was detected using the purified antibody. Western blot of ST8SIA4 Antibody in mouse spleen tissue lysates (35 ug/lane). ST8SIA4 (arrow) was detected using the purified antibody.
Western blot of ST8SIA4 Antibody in HL-60 cell line lysates (35 ug/lane). ST8SIA4 (arrow) was detected using the purified antibody. Western blot of ST8SIA4 Antibody in mouse spleen tissue lysates (35 ug/lane). ST8SIA4 (arrow) was detected using the purified antibody.

Flow Cytometry

ST8SIA4 Antibody flow cytometry of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
ST8SIA4 Antibody flow cytometry of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

ST8SIA4 Antibody IHC of formalin-fixed and paraffin-embedded brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
ST8SIA4 Antibody IHC of formalin-fixed and paraffin-embedded brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

Western blot of ST8SIA4 Antibody in HL-60 cell line lysates (35 ug/lane). ST8SIA4 (arrow) was detected using the purified antibody. Western blot of ST8SIA4 Antibody in mouse spleen tissue lysates (35 ug/lane). ST8SIA4 (arrow) was detected using the purified antibody.
Western blot of ST8SIA4 Antibody in HL-60 cell line lysates (35 ug/lane). ST8SIA4 (arrow) was detected using the purified antibody. Western blot of ST8SIA4 Antibody in mouse spleen tissue lysates (35 ug/lane). ST8SIA4 (arrow) was detected using the purified antibody.

Flow Cytometry

ST8SIA4 Antibody flow cytometry of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
ST8SIA4 Antibody flow cytometry of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

ST8SIA4 Antibody IHC of formalin-fixed and paraffin-embedded brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
ST8SIA4 Antibody IHC of formalin-fixed and paraffin-embedded brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

Western blot of ST8SIA4 Antibody in HL-60 cell line lysates (35 ug/lane). ST8SIA4 (arrow) was detected using the purified antibody. Western blot of ST8SIA4 Antibody in mouse spleen tissue lysates (35 ug/lane). ST8SIA4 (arrow) was detected using the purified antibody.
Western blot of ST8SIA4 Antibody in HL-60 cell line lysates (35 ug/lane). ST8SIA4 (arrow) was detected using the purified antibody. Western blot of ST8SIA4 Antibody in mouse spleen tissue lysates (35 ug/lane). ST8SIA4 (arrow) was detected using the purified antibody.

Flow Cytometry

ST8SIA4 Antibody flow cytometry of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
ST8SIA4 Antibody flow cytometry of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.


Requested From: 
Date Requested: 6/20/2018

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