Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human, Mouse (tested or 100% immunogen sequence identity)
Protein A purified
IHC - Frozen
Specificity and Use
SSEA-4 antibody was raised against human embryonal carcinoma cell line 2102 Ep.
Recognizes a carbohydrate epitope of human SSEA-4. Species cross-reactivity: Mouse.
Flow Cytometry, Immunohistochemistry. Immunohistochemistry: This antibody has been used with the appropriate secondary reagents at a concentration of 5-10 ug/mL in fixed cells. Cells were fixed with 4% paraformaldehyde in PBS at room temperature for 20 minutes, then blocked with PBS containing 10% normal donkey serum and 1% BSA at room temperature for 45 minutes. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by Rhodamine Red coupled anti-mouse IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1% BSA. This antibody can also be used in unfixed, shock frozen tissue at a concentration of 10 ug/mL. Flow Cytometry: Dilute this antibody to 0.1 mg/mL and add 5 ul of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 ul of a 25 ug/mL stock solution of a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome.
Lyophilized from TBS, 5% trehalose. Reconstitution: Reconstitute, sterile PBS. If 0.2 ml of PBS is used, the antibody concentration will be 500 ug/ml.