Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human (tested or 100% immunogen sequence identity)
Ammonium sulfate precipitation
IHC - Paraffin
Specificity and Use
U937 histiocytic cell line.
Recognizes human CD15.
Suitable for use in Flow Cytometry and Immunohistochemistry. Immunohistochemistry (Formalin/paraffin): 2-4 ug/ml for 30 min at RT. Staining of formalin/paraffin tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Staining is further enhanced if heat-induced epitope retrieval is followed by digestion of tissue sections with pepsin (1 mg/ml) for only 2 min at RT. Positive control: Reed-Sternberg's cells in Hodgkin's lymphoma.
10 mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
For research use only.
About SSEA-1 / Lewis x / CD15
Also known as CD15, Lewis x, and 3-fucosyl-N-acetyl-lactosamine. It is synthezised by Fucosyltransferase 4 (FUT4).