Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone Bu28] (IgM) to Human SSEA-1 / Lewis x / CD15
IHC - Paraffin, Flow Cytometry
Human SSEA-1 / Lewis x / CD15
Human (tested or 100% immunogen sequence identity)
IgM Monoclonal [Bu28]
Ammonium sulfate precipitation
IHC - Paraffin
Flow Cytometry (1:100 - 1:200)
Specificity and Use
Recognizes human CD15, otherwise known as Lewis X and stage-specific embryonic antigen-1 (SSEA-1). The CD15 antigen is itself a terminal trisaccharide, Gal-beta1-4 (Fuc-alpha1-3) GlcNAc (3-FAL), which is found on a variety of glycoproteins and glycolipids. CD15 is predominately expressed by peripheral blood granulocytes and various epithelial tissues. It plays an important role in cell adhesion and has been used as a marker for diagnosis of Hodgkin's lymphoma.
Flow Cytometry: Use 10 ul of the suggested working dilution to label 1x10^6 cells in 100 ul. Method sheets are available on request.
PBS, pH 7.4, 0.09% sodium azide.
Long term: -20°C; Short term: -20°C
For research use only.
About SSEA-1 / Lewis x / CD15
Also known as CD15, Lewis x, and 3-fucosyl-N-acetyl-lactosamine. It is synthezised by Fucosyltransferase 4 (FUT4).