Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone Bu28] (IgM) to Human SSEA-1 / Lewis x / CD15
Human SSEA-1 / Lewis x / CD15
Human (tested or 100% immunogen sequence identity)
IgM Monoclonal [Bu28]
Ammonium sulfate precipitation
Flow Cytometry (1:1 - 1:5)
Specificity and Use
Recognizes human CD15, otherwise known as Lewis X and stage-specific embryonic antigen-1 (SSEA-1). The CD15 antigen is itself a terminal trisaccharide, Gal-beta1-4 (Fuc-alpha1-3) GlcNAc (3-FAL), which is found on a variety of glycoproteins and glycolipids. CD15 is predominately expressed by peripheral blood granulocytes and various epithelial tissues. It plays an important role in cell adhesion and has been used as a marker for diagnosis of Hodgkin's lymphoma.
Flow Cytometry: Use 10 ul of the suggested working dilution to label 1x10^6 cells in 100 ul. Method sheets are available on request. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
PBS, pH 7.4, 0.09% sodium azide, 1% BSA.
Long term: -20°C; Short term: -20°C
For research use only.
About SSEA-1 / Lewis x / CD15
Also known as CD15, Lewis x, and 3-fucosyl-N-acetyl-lactosamine. It is synthezised by Fucosyltransferase 4 (FUT4).