Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
SSB Antibody, Autoantigen La Antibody, La Antibody, La ribonucleoprotein Antibody, Lupus La antigen Antibody, SS-B Antibody, SS-B/La protein Antibody, La autoantigen Antibody, LARP3 Antibody, Lupus La protein Antibody
SSB / La is involved in diverse aspects of RNA metabolism, including binding and protecting poly(U) termini of nascent RNA polymerase III transcripts from exonuclease digestion, processing 5' and 3' ends of pre-tRNA precursors, acting as an RNA chaperone, and binding viral RNAs associated with hepatitis C virus. Autoantibodies reacting with this protein are found in the sera of patients with Sjogren syndrome and systemic lupus erythematosus.
IHC of paraffin-embedded lung tissue using anti-SSB mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded prostate tissue using anti-SSB mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded endometrium tissue using anti-SSB mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Ovary tissue using anti-SSB mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded breast tissue using anti-SSB mouse monoclonal antibody. (Dilution 1:50).
Immunofluorescent staining of HT29 cells using anti-SSB mouse monoclonal antibody.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-SSB monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SSB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SSB.
Immunoprecipitation(IP) of SSB by using monoclonal anti-SSB antibodies (Negative control: IP without adding anti-SSB antibody.). For each experiment, 500ul of DDK tagged SSB overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-SSB antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
Flow cytometry of Jurkat cells, using anti-SSB antibody (Red), compared to a nonspecific negative control antibody (Blue).