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Catalog Number Size Price
LS-B10046-50 50 µl (1 mg/ml) $460 
SSB / La Antibody - Anti-SSB / La antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
SSB / La Antibody - Immunofluorescent staining of HepG2 cells using anti-SSB mouse monoclonal antibody.
SSB / La Antibody - Anti-SSB mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SSB.
SSB / La Antibody - Western blot of extracts (35 ug) from 9 different cell lines by using anti-SSB monoclonal antibody.
SSB / La Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SSB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SSB.
SSB / La Antibody - Immunoprecipitation(IP) of SSB by using monoclonal anti-SSB antibodies (Negative control: IP without adding anti-SSB antibody.). For each experiment, 500ul of DDK tagged SSB overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-SSB antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
SSB / La Antibody - Flow cytometry of Jurkat cells, using anti-SSB antibody (Red), compared to a nonspecific negative control antibody (Blue).
SSB / La Antibody - Anti-SSB / La antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
SSB / La Antibody - Immunofluorescent staining of HepG2 cells using anti-SSB mouse monoclonal antibody.
SSB / La Antibody - Anti-SSB mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SSB.
SSB / La Antibody - Western blot of extracts (35 ug) from 9 different cell lines by using anti-SSB monoclonal antibody.
SSB / La Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SSB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SSB.
SSB / La Antibody - Immunoprecipitation(IP) of SSB by using monoclonal anti-SSB antibodies (Negative control: IP without adding anti-SSB antibody.). For each experiment, 500ul of DDK tagged SSB overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-SSB antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
SSB / La Antibody - Flow cytometry of Jurkat cells, using anti-SSB antibody (Red), compared to a nonspecific negative control antibody (Blue).
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IHC‑plus™ Monoclonal Mouse anti‑Human SSB / La Antibody (clone 1E11, IHC, IF, WB) LS‑B10046

IHC‑plus™ Monoclonal Mouse anti‑Human SSB / La Antibody (clone 1E11, IHC, IF, WB) LS‑B10046

Note: This antibody replaces LS-C114512
Antibody:
SSB / La Mouse anti-Human Monoclonal (1E11) Antibody
Application:
IHC, IHC-P, IF, WB, IP, Flo
Reactivity:
Human, Monkey, Mouse, Rat, Dog
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B10046-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
SSB / La Mouse anti-Human Monoclonal (1E11) Antibody
Application:
IHC, IHC-P, IF, WB, IP, Flo
Reactivity:
Human, Monkey, Mouse, Rat, Dog
Format:
Unconjugated, Unmodified

Specifications

Description
La antibody LS-B10046 is an unconjugated mouse monoclonal antibody to La (SSB) from human. It is reactive with human, mouse, rat and other species. Validated for Flow, IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human SSB / La
Synonyms
SSB | Autoantigen La | La | La ribonucleoprotein | Lupus La antigen | SS-B | SS-B/La protein | La autoantigen | LARP3 | Lupus La protein
Host
Mouse
Reactivity
Human, Monkey, Mouse, Rat, Dog (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal
Clone
1E11
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Immunogen
Protein expressed in 293T cell transfected with human SSB expression vector.
Specificity
Human SSB / La
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Immunofluorescence (1:50 - 1:100)
  • Western blot (1:1000 - 1:2000)
  • Immunoprecipitation
  • Flow Cytometry (1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% BSA
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SSB / La
P05455 NM_003142 NP_003133.1

Validation

SSB / La Antibody - Anti-SSB / La antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-SSB / La antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-SSB / La antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

LSBio Ratings

IHC-plus™ SSB / La Antibody (clone 1E11) for IHC, IF/Immunofluorescence, WB/Western, IP, Flow LS-B10046 has an LSBio Rating of
Laboratory Validation Score (4)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/19/2024