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| Catalog Number | Size | Price |
|---|---|---|
| LS-B2340-50 | 50 µg (0.5 mg/ml) | $485 |
IHC‑plus™ Monoclonal Mouse anti‑Human SRSF1 / SF2 Antibody (N‑Terminus, IHC, IF, WB) LS‑B2340
IHC‑plus™ Monoclonal Mouse anti‑Human SRSF1 / SF2 Antibody (N‑Terminus, IHC, IF, WB) LS‑B2340
Note: This antibody replaces LS-C17896
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Specifications
Description
SF2 antibody LS-B2340 is an unconjugated mouse monoclonal antibody to SF2 (SRSF1) (N-Terminus) from human. It is reactive with human, mouse and rat. Validated for IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human SRSF1 / SF2
Synonyms
SRSF1 | Alternative-splicing factor 1 | ASF | SF2 | SF2p33 | SFRS1 | Splicing factor 2 | ASF-1 | SR splicing factor 1 | SRp30a
Host
Mouse
Reactivity
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Clonality
IgG2b
Monoclonal
Purification
Protein A purified
Modifications
Unmodified
Immunogen
Recombinant SF2/ASF protein.
Epitope
N-Terminus
Specificity
Recognizes the N-terminal portion (aa1-97) of the SF2/ASF protein comprising the RNA-recognition motif (RRM1). Reactivity is not affected by phosphorylation state of SF2/ASF. Species cross-reactivity: Human (HeLa cells), mouse and rat. Cross-reactivity expected with other species because SF2/ASF is highly conserved.
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Immunofluorescence
- Western blot (0.5 - 1 µg/ml)
- Immunoprecipitation
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B2340 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2340 was determined to be 5 ug/ml.
Presentation
PBS, pH 7.4, 0.09% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About SRSF1 / SF2
Validation
Anti-SF2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-SF2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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LSBio Ratings
IHC-plus™ SRSF1 / SF2 Antibody (N-Terminus) for IHC, IF/Immunofluorescence, WB/Western, IP LS-B2340 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (1)
Metformin decreases progerin expression and alleviates pathological defects of Hutchinson-Gilford progeria syndrome cells. Egesipe AL, Blondel S, Cicero AL, Jaskowiak AL, Navarro C, Sandre-Giovannoli A, Levy N, Peschanski M, Nissan X. NPJ aging and mechanisms of disease. 2017 November;2:16026. (Human)
Customer Reviews (0)
Featured Products
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, Flow Cytometry
Species:
Human, Chimpanzee, Orangutan, Gibbon, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Pig, Xenopus
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Monkey, Mouse, Rat
Applications:
IHC, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/18/2024
Date Requested: 4/18/2024
