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Anti-SRSF1 / SF2 Antibody (aa158-186) LS-C169019

Ordering

Wt. Vol. Conc. Price
- 400 µl - $305
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular SRSF1 / SF2 Antibodies

Anti-SRSF1 / SF2 Antibody (N-Terminus) IHC-plus™ LS-B2340
Mouse Monoclonal (IgG2b) to Human SRSF1 / SF2
Human, Mouse, Rat
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry Image
Anti-SRSF1 / SF2 Antibody (aa158-186) LS-C169019
Rabbit Polyclonal to Human SRSF1 / SF2
Human
IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human SRSF1 / SF2
Human
IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated

Details

Human SRSF1 / SF2
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Ammonium sulfate precipitation
Unmodified

Applications

  • IHC - Paraffin (1:50 - 1:100)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)

Specificity and Use

SRSF1 / SF2 antibody was raised against kLH-conjugated synthetic peptide between amino acids 158-186 from C-terminal region of human SFRS1.
LS-E8506 - Lyophilized - 100 µg - $145.00
aa158-186
Human SFRS1 / SF2

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About SRSF1 / SF2

Q07955 NM_006924 NP_008855.1

SRSF1 Antibody, Alternative-splicing factor 1 Antibody, ASF Antibody, SF2 Antibody, SF2p33 Antibody, SFRS1 Antibody, Splicing factor 2 Antibody, ASF-1 Antibody, SR splicing factor 1 Antibody, SRp30a Antibody

Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5'-splice site-containing pre-mRNA. Binds to purine-rich RNA sequences, either the octamer, 5'-RGAAGAAC-3' (r=A or G) or the decamers, AGGACAGAGC/AGGACGAAGC.

Immunohistochemistry

Immunohistochemistry
Formalin-fixed and paraffin-embedded human colon carcinoma reacted with SFRS1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot

Western blot
Western blot of SFRS1 (arrow) using rabbit polyclonal SFRS1 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SFRS1 gene.

Western blot

Western blot
Western blot of SFRS1 Antibody in HL-60 cell line lysates (35 ug/lane). SFRS1 (arrow) was detected using the purified antibody.

Flow Cytometry

Flow Cytometry
SFRS1 Antibody flow cytometry of WiDr cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Requested From: United States
Date Requested: 12/2/2016

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