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SRP68 Antibody (aa36‑85) LS‑C101869 Best Seller
SRP68 antibody LS-C101869 is an unconjugated rabbit polyclonal antibody to SRP68 from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C101869-50
50 µg
Unavailable

Popular SRP68 Products

Immunofluorescence of monoclonal antibody to SRP68 on HeLa cell . [antibody concentration 10 ug/ml]
Species: Human
Applications: Immunofluorescence, Western blot, ELISA
Detection of Human SRP68 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SRP68 antibody used for IP at 6 ug/mg lysate. SRP68 was also immunoprecipitated by rabbit anti-SRP68 antibody BL14251. For blotting immunoprecipitated SRP68, was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Species: Human, Mouse, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit
Applications: Western blot, Immunoprecipitation
Immunohistochemistry of Human brain using SRP68 Polyclonal Antibody at dilution of 1:25.
Species: Human, Mouse
Applications: IHC, ELISA
Immunohistochemistry of Human brain using SRP68 Polyclonal Antibody at dilution of 1:25.
Species: Human, Mouse
Applications: IHC, ELISA

Product Description

SRP68 antibody LS-C101869 is an unconjugated rabbit polyclonal antibody to SRP68 from human, mouse, rat and other species. Validated for WB.
About SRP68
SRP68 is a subunit of the signal recognition particle (SRP). The SRP is a ribonucleoprotein complex that transports secreted and membrane proteins to the endoplasmic reticulum for processing. The complex includes a 7S RNA and six protein subunits. The encoded protein is the 68kDa component of the SRP, and forms a heterodimer with the 72kDa subunit that is required for SRP function. Q9UHB9 NM_014230 NP_055045.2

SRP68 Antibody, Signal recognition particle 68 Antibody


Specifications

Target
Human SRP68
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse, Pig, Rabbit, Chicken, Zebrafish (tested or 100% immunogen sequence identity)
Predicted
Guinea pig, Xenopus (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with HRP, FITC, Biotin.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
SRP68 antibody was raised against synthetic peptide located between aa36-85 of human SRP68 (Q9UHB9, NP_055045). Percent identity by BLAST analysis: Human, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Turkey, Chicken, Lizard (100%); Elephant, Guinea pig, Platypus, Xenopus, Stickleback (92%); Zebrafish, Drosophila, Beetle (85%).
Blocking Peptide
LS-E14936 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C101869. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa36-85
Specificity
Human SRP68
Usage
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

SRP68 antibody LS-C101869 Western blot of 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SRP68 antibody LS-C101869 Western blot of 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

SRP68 antibody LS-C101869 Western blot of 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SRP68 antibody LS-C101869 Western blot of 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

SRP68 antibody LS-C101869 Western blot of 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SRP68 antibody LS-C101869 Western blot of 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

SRP68 antibody LS-C101869 Western blot of 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SRP68 antibody LS-C101869 Western blot of 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/24/2018

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