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Anti-SRC Antibody (clone 5A18) IHC-plus™ LS-B3266

Note: This antibody replaces LS-C63087

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular SRC Antibodies

Anti-SRC Antibody (clone 5A18) IHC-plus™ LS-B3266
Mouse Monoclonal [clone 5A18] to Human SRC
Human, Mouse, Rat
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-SRC Antibody (aa1-30) IHC-plus™ LS-B3308
Rabbit Polyclonal to Human SRC
Human, Mouse
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed Publications Publications (1)
Mouse Monoclonal [clone 5A18] to Human SRC
Human, Mouse, Rat
IHC - Paraffin, Western blot
Unconjugated

Details

Human SRC
Mouse
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Monoclonal [5A18]
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (20 µg/ml)
  • Western blot

Specificity and Use

SRC antibody was raised against recombinant full length human c-Src.
Human, mouse, and rat
Immunohistochemistry: LS-B3266 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3266 was determined to be 20 ug/ml.

Packaging

PBS, pH 7.2, 0.09% azide, 50% glycerol.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

Publications (1)

1
Src blockage by siRNA inhibits VEGF-induced vascular hyperpemeability and osteoclast activity - an in vitro mechanism study for preventing destructive repair of osteonecrosis. Cao HJ, Zheng LZ, Wang N, Wang LY, Li Y, Li D, Lai YX, Wang XL, Qin L. Bone. May;74:58-68. [Full Text Article] [PubMed:25554601]

About SRC

P12931 NM_005417 NP_005408.1

SRC Antibody, ASV Antibody, Pp60c-src Antibody, Tyrosine kinase pp60c-src Antibody, Tyrosine-protein kinase SRC-1 Antibody, v-src Antibody, C-SRC Antibody, p60-Src Antibody, Proto-oncogene c-Src Antibody, SRC1 Antibody

Non-receptor protein tyrosine kinase which is activated following engagement of many different classes of cellular receptors including immune response receptors, integrins and other adhesion receptors, receptor protein tyrosine kinases, G protein-coupled receptors as well as cytokine receptors.

Immunohistochemistry

Immunohistochemistry
Anti-SRC antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3266 concentration 20 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-SRC antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3266 concentration 20 ug/ml.

Western blot

Western blot
Lane1: MW Marker, Lane 2: HeLa, Lane 3:3T3, Lane 4: PC12.
 

Requested From: United States
Date Requested: 12/6/2016

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