Bovine SR Proteins

Mouse

Bovine, Human, Mouse, Rat, Rabbit (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Protein A purified

Unmodified

Purified, de-phosphorylated bovine Srp55 and Drosophila p55-GST.

Detects a sub-set of the non-snRNP splicing factors termed SR proteins. The antibody detects SRp75, SRp55, SRp40 and SRp20 but not SRp30a or b (ASF/SF2, SC-35) proteins. Recognizes approximately 20 distinct nuclear proteins including the U1 70K component of the U1 snRNP and both subunits of U2AF1. Species cross-reactivity (Western Blot): chicken, human, mouse, rabbit, canine, rat, Xenopus. Reactivity has not been detected with yeasts.

• IHC
• IHC - Paraffin (10 µg/ml)
• Immunofluorescence (0.5 - 2 µg/ml)
• Western blot (3 - 5 µg/ml)
• ELISA (0.1 - 1 µg/ml)

Suitable for use in Immunofluorescence, ELISA, Western Blot. Reactivity is confirmed by Western Blot. ELISA: 0.1-1 ug/ml. Immunofluorescence: 0.5-2 ug/ml, human. Western Blot: 3-5 ug/ml. Positive control: HeLa cell, nuclear extracts.

PBS, pH 7.2, 0.1% Sodium Azide

May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for up to 1 year.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.