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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SQSTM1 Antibody (clone 5A1) LS‑C172546
SQSTM1 antibody LS-C172546 is an unconjugated mouse monoclonal antibody to human SQSTM1. Validated for Flow, IF, IHC and WB.
Catalog
Size
Price
LS-C172546-100
100 µl (0.96 mg/ml)
Unavailable

Popular SQSTM1 Products

Antibody
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Anti-SQSTM1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5839 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Product Description

SQSTM1 antibody LS-C172546 is an unconjugated mouse monoclonal antibody to human SQSTM1. Validated for Flow, IF, IHC and WB.
About SQSTM1
Autophagy receptor that interacts directly with both the cargo to become degraded and an autophagy modifier of the MAP1 LC3 family. Required both for the formation and autophagic degradation of polyubiquitin-containing bodies, called ALIS (aggresome-like induced structures) and links ALIS to the autophagic machinery. Involved in midbody ring degradation. May regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. Q13501 NM_003900 NP_003891.1

SQSTM1 Antibody, A170 Antibody, ORCA Antibody, OSIL Antibody, Paget disease of bone 3 Antibody, p60 Antibody, p62 Antibody, PDB3 Antibody, Sequestosome-1 Antibody, Ubiquitin-binding protein p62 Antibody, Oxidative stress induced like Antibody, p62B Antibody, ZIP3 Antibody, Sequestosome 1 Antibody, EBI3-associated protein p60 Antibody, EBIAP Antibody


Specifications

Target
Human SQSTM1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal [5A1]
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:150)
  • Immunofluorescence (1:100)
  • Western blot (1:2000)
  • Flow Cytometry (1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
SQSTM1 antibody was raised against full length human recombinant protein of human SQSTM1 (NP_003891) produced in HEK293T cell.
Specificity
Human P62 / SQSTM1
Presentation
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

IHC of paraffin-embedded Human prostate tissue using anti-SQSTM1 mouse monoclonal antibody.
IHC of paraffin-embedded Human prostate tissue using anti-SQSTM1 mouse monoclonal antibody.

Immunofluorescence

Anti-SQSTM1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SQSTM1.
Anti-SQSTM1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SQSTM1.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SQSTM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SQSTM1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SQSTM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SQSTM1.

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SQSTM1 antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SQSTM1 antibody, and then analyzed by flow cytometry.

Immunohistochemistry

IHC of paraffin-embedded Human prostate tissue using anti-SQSTM1 mouse monoclonal antibody.
IHC of paraffin-embedded Human prostate tissue using anti-SQSTM1 mouse monoclonal antibody.

Immunofluorescence

Anti-SQSTM1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SQSTM1.
Anti-SQSTM1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SQSTM1.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SQSTM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SQSTM1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SQSTM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SQSTM1.

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SQSTM1 antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SQSTM1 antibody, and then analyzed by flow cytometry.

Immunohistochemistry

IHC of paraffin-embedded Human prostate tissue using anti-SQSTM1 mouse monoclonal antibody.
IHC of paraffin-embedded Human prostate tissue using anti-SQSTM1 mouse monoclonal antibody.

Immunofluorescence

Anti-SQSTM1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SQSTM1.
Anti-SQSTM1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SQSTM1.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SQSTM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SQSTM1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SQSTM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SQSTM1.

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SQSTM1 antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SQSTM1 antibody, and then analyzed by flow cytometry.

Immunohistochemistry

IHC of paraffin-embedded Human prostate tissue using anti-SQSTM1 mouse monoclonal antibody.
IHC of paraffin-embedded Human prostate tissue using anti-SQSTM1 mouse monoclonal antibody.

Immunofluorescence

Anti-SQSTM1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SQSTM1.
Anti-SQSTM1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SQSTM1.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SQSTM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SQSTM1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SQSTM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SQSTM1.

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SQSTM1 antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SQSTM1 antibody, and then analyzed by flow cytometry.


Requested From: 
Date Requested: 6/21/2018

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