Human SPY1 / SPDYA

Rabbit

Human (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Immunoaffinity chromatography

Unmodified

Recombinant full length protein of human Speedy A

Recognizes endogenous levels of Speedy A protein.

• ICC
• Immunofluorescence (1:50 - 1:200)
• Western blot (1:500 - 1:2000)

Western blot analysis of Speedy A expression in PC3 (A), U87 (B) whole cell lysates. Immunofluorescent analysis of Speedy A staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q5MJ70 NCBI: NM_182756 NP_877433.2