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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SPTLC2 / LCB2 Antibody (aa72‑121) LS‑C102311
LCB2 antibody LS-C102311 is an unconjugated rabbit polyclonal antibody to LCB2 (SPTLC2) from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C102311-50
50 µg
Unavailable

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Western blot of SPTLC2 / LCB2 antibody.
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Western blot of SPTLC2 / LCB2 antibody.
Species: Mouse
Applications: IHC, Western blot

Product Description

LCB2 antibody LS-C102311 is an unconjugated rabbit polyclonal antibody to LCB2 (SPTLC2) from human, mouse, rat and other species. Validated for WB.
About SPTLC2 / LCB2
Serine palmitoyltransferase (SPT). The heterodimer formed with LCB1/SPTLC1 constitutes the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC2-SPTSSA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC2-SPTSSB complex displays a preference for C18-CoA substrate. O15270 NM_004863 NP_004854.1

SPTLC2 Antibody, HSN1C Antibody, HLCB2a Antibody, KIAA0526 Antibody, LCB2 Antibody, LCB2A Antibody, SPT 2 Antibody, NSAN1C Antibody, SPT2 Antibody, LCB 2 Antibody, Serine palmitoyltransferase 2 Antibody


Specifications

Target
Human SPTLC2 / LCB2
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Guinea pig, Hamster, Rabbit, Xenopus, Zebrafish (tested or 100% immunogen sequence identity)
Predicted
Bat, Dog, Horse, Pig (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with HRP, Biotin, FITC.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
SPTLC2 / LCB2 antibody was raised against synthetic peptide located between aa72-121 of human SPTLC2 (O15270, NP_004854). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Bovine, Rabbit, Opossum, Guinea pig, Platypus, Xenopus, Zebrafish (100%); Bat, Horse (92%); Panda, Dog, Pig, Zebra finch, Stickleback (90%); Lizard (84%).
Blocking Peptide
LS-E17589 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C102311. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa72-121
Specificity
Human SPTLC2
Usage
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

SPTLC2 antibody LS-C102311 Western blot of HepG2 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SPTLC2 antibody LS-C102311 Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

SPTLC2 antibody LS-C102311 Western blot of HepG2 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SPTLC2 antibody LS-C102311 Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

SPTLC2 antibody LS-C102311 Western blot of HepG2 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SPTLC2 antibody LS-C102311 Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

SPTLC2 antibody LS-C102311 Western blot of HepG2 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SPTLC2 antibody LS-C102311 Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/19/2018

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