Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human, Bovine (tested or 100% immunogen sequence identity)
Monkey, Mouse, Rat, Dog (at least 90% immunogen sequence identity)
IHC - Paraffin (2.5 - 5 µg/ml)
Specificity and Use
SPTBN4 antibody was raised against synthetic peptide AQVPGEVDNMEGL-C from positions 2-14 of human SPTBN4 (Q9H254). Percent identity by BLAST analysis: Human, Gorilla, Panda, Bovine (100%); Monkey, Marmoset, Mouse, Rat, Dog (92%); Hamster (85%).
Polarization of membrane associated proteins in the choroid plexus epithelium from normal and slc4a10 knockout mice. Christensen IB, Gyldenholm T, Damkier HH, Praetorius J. Frontiers in physiology. 2013 4:344. (IHC; Mouse)[Full Text Article]
Spectrin is an actin crosslinking and molecular scaffold protein that links the plasma membrane to the actin cytoskeleton, and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. It is composed of two antiparallel dimers of alpha- and beta- subunits. This gene is one member of a family of beta-spectrin genes. The encoded protein localizes to the nuclear matrix, PML nuclear bodies, and cytoplasmic vesicles.