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Catalog Number Size Price
LS-B10415-50 50 µl $460 
SPTAN1 / Alpha Fodrin Antibody - Anti-SPTAN1 / Alpha Fodrin antibody IHC staining of human small intestine, submucosal plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
SPTAN1 / Alpha Fodrin Antibody - Mixed neuron-glial cultures stained with rabbit antibody to alpha-II spectrin (green) and mouse polyclonal antibody to Nestin (red). The alpha-II spectrin antibody stains numerous axonal and dendritic profiles in these cultures, clearly revealing the submembranous cytoskeleton. Since alpha-II spectrin is specific for neurons in the CNS, the glial cells in this culture are not recognized by this antibody.
SPTAN1 / Alpha Fodrin Antibody - Western blots of mouse sciatic nerve extract (lanes 1 and 2) and brain homogenate (3). A prominent band running at 240 kDa represents the intact alpha-II spectrin heavy chain. More minor bands below this likely represent in vivo proteolytic fragments of alpha-II spectrin.
SPTAN1 / Alpha Fodrin Antibody - Anti-SPTAN1 / Alpha Fodrin antibody IHC staining of human small intestine, submucosal plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
SPTAN1 / Alpha Fodrin Antibody - Mixed neuron-glial cultures stained with rabbit antibody to alpha-II spectrin (green) and mouse polyclonal antibody to Nestin (red). The alpha-II spectrin antibody stains numerous axonal and dendritic profiles in these cultures, clearly revealing the submembranous cytoskeleton. Since alpha-II spectrin is specific for neurons in the CNS, the glial cells in this culture are not recognized by this antibody.
SPTAN1 / Alpha Fodrin Antibody - Western blots of mouse sciatic nerve extract (lanes 1 and 2) and brain homogenate (3). A prominent band running at 240 kDa represents the intact alpha-II spectrin heavy chain. More minor bands below this likely represent in vivo proteolytic fragments of alpha-II spectrin.
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IHC‑plus™ Polyclonal Rabbit anti‑Human SPTAN1 / Alpha Fodrin Antibody (IHC, IF, WB) LS‑B10415

IHC‑plus™ Polyclonal Rabbit anti‑Human SPTAN1 / Alpha Fodrin Antibody (IHC, IF, WB) LS‑B10415

Note: This antibody replaces LS-C204578
Antibody:
SPTAN1 / Alpha Fodrin Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B10415-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
SPTAN1 / Alpha Fodrin Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
Alpha Fodrin antibody LS-B10415 is an unconjugated rabbit polyclonal antibody to Alpha Fodrin (SPTAN1) from human. It is reactive with human and mouse. Validated for ICC, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human SPTAN1 / Alpha Fodrin
Synonyms
SPTAN1 | Alpha-II spectrin | Alpha Fodrin | Fodrin alpha chain | EIEE5 | NEAS | Alpha-fodrin | Spectrin alpha chain, brain | SPTA2
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Antiserum
Modifications
Unmodified
Immunogen
Mixture of five recombinant constructs containing the entire C-Terminus of human alpha-II spectrin (aa 676-2400). The 9th spectrin repeat also includes a Src-homology 3 domain, and the extreme C-Terminus beyond the 20th spectrin repeat contains two EF hand calcium binding motifs. These constructs were expressed in and purified from E. coli.
Specificity
Human SPTAN1 / Alpha Fodrin
Applications
  • IHC
  • IHC - Paraffin (1:100)
  • ICC (1:500 - 1:1000)
  • Immunofluorescence (1:500 - 1:1000)
  • Western blot (1:5000 - 1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
The serum can be diluted to 1:500-1:1000 for immunofluorescence staining and 1:5000-1:10000 for western blotting. On western blots look for a major band at 240kDa, depending on the species.
Presentation
Antiserum
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SPTAN1 / Alpha Fodrin
Q13813 NM_003127 NP_003118.2

Validation

SPTAN1 / Alpha Fodrin Antibody - Anti-SPTAN1 / Alpha Fodrin antibody IHC staining of human small intestine, submucosal plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-SPTAN1 / Alpha Fodrin antibody IHC staining of human small intestine, submucosal plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-SPTAN1 / Alpha Fodrin antibody IHC staining of human small intestine, submucosal plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

LSBio Ratings

IHC-plus™ SPTAN1 / Alpha Fodrin Antibody for IHC, ICC, IF/Immunofluorescence, WB/Western LS-B10415 has an LSBio Rating of
Laboratory Validation Score (4)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/25/2024