Sprouty 2 antibody LS-C10336 is an unconjugated rabbit polyclonal antibody to human Sprouty 2 (SPRY2). Validated for ELISA, IP and WB.
Human SPRY2 / Sprouty 2
(tested or 100% immunogen sequence identity)
- Western blot (1:2000)
His6X tagged full-length human Sprouty-2 protein.
Reacts with human Sprouty-2 protein (38kD). Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.
Suitable for use in ELISA, Western Blot and Immunoprecipitation. Western Blot: 1:2000; A 38kD band corresponding to human Sprouty-2 is observed. Detection occurs using HRP Goat anti-Rabbit IgG and ECL. Immunoprecipitation: 20 ul of antibody is used to immunoprecipitate hSpry2 protein from 1 mg of a HeLa cell lysate in 1ml. Incubate the antibody for 2hr with the cell lysate. Immunoprecipitate the complex with Protein G agarose.
PBS, pH 7.2, 0.01% Sodium Azide, No stabilizing proteins added
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.