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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SPRY2 / Sprouty 2 Antibody LS‑C10336
Sprouty 2 antibody LS-C10336 is an unconjugated rabbit polyclonal antibody to human Sprouty 2 (SPRY2). Validated for ELISA, IP and WB.
Antibody
Catalog
Size
Price
LS-C10336-100
100 µg
$740

Popular SPRY2 / Sprouty 2 Products

Anti-SPRY2 / Sprouty 2 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Immunoperoxidase of monoclonal antibody to SPRY2 on formalin-fixed paraffin-embedded human lymphoma. [antibody concentration 3 ug/ml].
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Anti-SPRY2 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig, Rabbit
Applications: IHC, IHC - Paraffin, Western blot
Western blot of recombinant SPRY2 / Sprouty 2.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Mouse
Applications: IHC, Western blot
Immunohistochemistry of paraffin-embedded human brain tissue using SPRY2 Antibody at dilution of 1:100
Species: Human, Mouse
Applications: IHC, Immunofluorescence, Western blot, ELISA

Product Description

Sprouty 2 antibody LS-C10336 is an unconjugated rabbit polyclonal antibody to human Sprouty 2 (SPRY2). Validated for ELISA, IP and WB.

Specifications

Target
Human SPRY2 / Sprouty 2
Synonyms
SPRY2, HSPRY2, Protein sprouty homolog 2, Sprouty homolog 2 (Drosophila), Sprouty 2, Spry-2, Sprouty (Drosophila) homolog 2
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1:2000)
  • Immunoprecipitation
  • ELISA
Immunogen
His6X tagged full-length human Sprouty-2 protein.
Specificity
Reacts with human Sprouty-2 protein (38kD). Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.
Usage
Suitable for use in ELISA, Western Blot and Immunoprecipitation. Western Blot: 1:2000; A 38kD band corresponding to human Sprouty-2 is observed. Detection occurs using HRP Goat anti-Rabbit IgG and ECL. Immunoprecipitation: 20 ul of antibody is used to immunoprecipitate hSpry2 protein from 1 mg of a HeLa cell lysate in 1ml. Incubate the antibody for 2hr with the cell lysate. Immunoprecipitate the complex with Protein G agarose.
Presentation
PBS, pH 7.2, 0.01% Sodium Azide, No stabilizing proteins added
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only.
About SPRY2 / Sprouty 2
SPRY2 / Sprouty 2 is a protein belonging to the sprouty family. The encoded protein contains a carboxyl-terminal cysteine-rich domain essential for the inhibitory activity on receptor tyrosine kinase signaling proteins and is required for growth factor stimulated translocation of the protein to membrane ruffles. In primary dermal endothelial cells this gene is transiently upregulated in response to fibroblast growth factor two. O43597 NM_005842 NP_005833.1


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Requested From: United States
Date Requested: 11/15/2018

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