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Anti-SPRY2 / Sprouty 2 Antibody (aa26-39) LS-C10337

Ordering

Wt. Vol. Conc. Price
200 µg - - $515
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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Rabbit Polyclonal (IgG) to Human SPRY2 / Sprouty 2
Human, Mouse, Rat, Dog, Hamster, Pig
Western blot
Unconjugated

Details

Human SPRY2 / Sprouty 2
Rabbit
Human, Mouse, Rat, Dog, Hamster, Pig (tested or 100% immunogen sequence identity)
Monkey, Bovine, Horse (at least 90% immunogen sequence identity)
IgG Polyclonal
Protein A purified

Applications

Western blot (0.1 - 1 µg/ml)

Specificity and Use

SPRY2 / Sprouty 2 antibody was raised against kLH-conjugated, synthetic peptide corresponding to aa26-39 (C-GEPDPRDALTQQVH) of human Sprouty2, with an N-terminal cysteine added for conjugation purposes. The immunizing sequence is identical in rat and mouse. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Mouse, Rat, Hamster, Panda, Dog, Pig, Opossum (100%); Marmoset, Bovine, Horse (93%); Elephant, Rabbit, Turkey, Chicken, Platypus (86%).
aa26-39
Recognizes Sprouty2, Mr ~35-40kD. A non-specific protein was also detected, Mr ~70kD. Species cross-reactivity: Human and mouse. Cross-reactivity with rat predicted, based on sequence homology.
Suitable for use in Western Blot. Western Blot: 0.1-1 ug/ml detects Sprouty2 in RIPA lysates from HeLa and 3T3/A31 cells.

Packaging

0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05% sodium azide, 30% glycerol.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About SPRY2 / Sprouty 2

O43597 NM_005842 NP_005833.1

SPRY2 Antibody, HSPRY2 Antibody, Protein sprouty homolog 2 Antibody, Sprouty homolog 2 (Drosophila) Antibody, Sprouty 2 Antibody, Spry-2 Antibody, Sprouty (Drosophila) homolog 2 Antibody

SPRY2 / Sprouty 2 is a protein belonging to the sprouty family. The encoded protein contains a carboxyl-terminal cysteine-rich domain essential for the inhibitory activity on receptor tyrosine kinase signaling proteins and is required for growth factor stimulated translocation of the protein to membrane ruffles. In primary dermal endothelial cells this gene is transiently upregulated in response to fibroblast growth factor two.

Requested From: United States
Date Requested: 9/25/2016

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