Human SPRY2 / Sprouty 2
Human, Mouse, Rat, Dog, Hamster, Pig
(tested or 100% immunogen sequence identity)
Monkey, Bovine, Horse (at least 90% immunogen sequence identity)
Also available conjugated with AP
Protein A purified
- Western blot (0.1 - 1 µg/ml)
- (applications tested for the base form of this product only)
KLH-conjugated, synthetic peptide corresponding to aa26-39 (C-GEPDPRDALTQQVH) of human Sprouty2, with an N-terminal cysteine added for conjugation purposes. The immunizing sequence is identical in rat and mouse. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Mouse, Rat, Hamster, Panda, Dog, Pig, Opossum (100%); Marmoset, Bovine, Horse (93%); Elephant, Rabbit, Turkey, Chicken, Platypus (86%).
Recognizes Sprouty2, Mr ~35-40kD. A non-specific protein was also detected, Mr ~70kD. Species cross-reactivity: Human and mouse. Cross-reactivity with rat predicted, based on sequence homology.
Suitable for use in Western Blot. Western Blot: 0.1-1 ug/ml detects Sprouty2 in RIPA lysates from HeLa and 3T3/A31 cells.
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.