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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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SPRY2 / Sprouty 2 Antibody (aa26‑39) LS‑C10337
Antibody
Sprouty 2 antibody LS-C10337 is an unconjugated rabbit polyclonal antibody to Sprouty 2 (SPRY2) from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C10337-200
200 µg
Unavailable

Popular SPRY2 / Sprouty 2 Products

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Product Description

Sprouty 2 antibody LS-C10337 is an unconjugated rabbit polyclonal antibody to Sprouty 2 (SPRY2) from human, mouse, rat and other species. Validated for WB.
About SPRY2 / Sprouty 2
SPRY2 / Sprouty 2 is a protein belonging to the sprouty family. The encoded protein contains a carboxyl-terminal cysteine-rich domain essential for the inhibitory activity on receptor tyrosine kinase signaling proteins and is required for growth factor stimulated translocation of the protein to membrane ruffles. In primary dermal endothelial cells this gene is transiently upregulated in response to fibroblast growth factor two. O43597 NM_005842 NP_005833.1

SPRY2 Antibody, HSPRY2 Antibody, Protein sprouty homolog 2 Antibody, Sprouty homolog 2 (Drosophila) Antibody, Sprouty 2 Antibody, Spry-2 Antibody, Sprouty (Drosophila) homolog 2 Antibody


Specifications

Target
Human SPRY2 / Sprouty 2
Host
Rabbit
Reactivity
Human, Mouse, Rat, Dog, Hamster, Pig (tested or 100% immunogen sequence identity)
Predicted
Monkey, Bovine, Horse (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Western blot (0.1 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
KLH-conjugated, synthetic peptide corresponding to aa26-39 (C-GEPDPRDALTQQVH) of human Sprouty2, with an N-terminal cysteine added for conjugation purposes. The immunizing sequence is identical in rat and mouse. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Mouse, Rat, Hamster, Panda, Dog, Pig, Opossum (100%); Marmoset, Bovine, Horse (93%); Elephant, Rabbit, Turkey, Chicken, Platypus (86%).
Epitope
aa26-39
Specificity
Recognizes Sprouty2, Mr ~35-40kD. A non-specific protein was also detected, Mr ~70kD. Species cross-reactivity: Human and mouse. Cross-reactivity with rat predicted, based on sequence homology.
Usage
Suitable for use in Western Blot. Western Blot: 0.1-1 ug/ml detects Sprouty2 in RIPA lysates from HeLa and 3T3/A31 cells.
Presentation
0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05% sodium azide, 30% glycerol.
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: 
Date Requested: 6/24/2018

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