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Anti-Sprouty 4 / SPRY4 Antibody (aa306-322) IHC-plus™ LS-B77

Note: This antibody replaces LS-C19051

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395

LSBio (Direct) LSBio (Direct)
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Sprouty 4 / SPRY4 Antibody LS-B77 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-SPRY4 antibody IHC of human heart.
Western blot
Anti-Sprouty-4 Antibody - Western Blot.

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Rabbit Polyclonal to Human Sprouty 4 / SPRY4
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human Sprouty 4 / SPRY4
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Immunoaffinity purified

Applications

IHC - Paraffin (2.5 µg/ml), Western blot (1:100 - 1:1000), ELISA (1:3000 - 1:12000)

Specificity and Use

Sprouty 4 / SPRY4 antibody was raised against synthetic peptide from human SPRY4.
aa306-322
Amino acids 306-322 of Human Sprouty-4 protein.
Immunohistochemistry: LS-B77 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B77 was determined to be 2.5 ug/ml.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About Sprouty 4 / SPRY4

Q9C004 NM_030964 NP_112226.2

SPRY4 Antibody, Protein sprouty homolog 4 Antibody, Sprouty 4 Antibody, Sprouty homolog 4 (Drosophila) Antibody, Sprouty 4 Antibody, Spry-4 Antibody

Sprouty-4 (also known as SPRY4) is an inhibitor of the insulin receptor and EGFR-transduced mitogen-activated protein kinase (MAPK) signaling pathway. It is positioned upstream of RAS activation and impairs the formation of active GTP-RAS. Sprouty-4 is a cytoplasmic protein that interacts with the C-terminus of TESK1. Multiple splice variants have been reported for this protein.

Immunohistochemistry

Immunohistochemistry
Anti-SPRY4 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B77 concentration 5 ug/ml.

Western blot

Western blot
Anti-Sprouty-4 Antibody - Western Blot. Western blot of Affinity Purified anti-Sprouty-4 antibody shows detection of a doublet band ~35 kD corresponding to human Sprouty-4. Approximately 30 ug of HeLa (lane 1), SW13 (lane 2) and C2C12 (Lane 3) whole cell lysates were separated by 10% SDS-PAGE and transferred onto nitrocellulose. After blocking with 5% non-fat dry milk in TBST the membrane was probed with the primary antibody diluted to 1:100 overnight at 4C followed by washes and reaction with a 1:5000 dilution of HRP conjugated Gt-a-Rabbit IgG [H&L] MX (LS-C60865) for 1 h at room temperature. Signal was procebetaed by ECL (Pierce). Other detection systems will yield similar results.

Requested From: United States
Date Requested: 8/30/2015

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