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Anti-SPP1 / Osteopontin Antibody (N-Terminus) IHC-plus™ LS-B5626

Note: This antibody replaces LS-C110191

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl 1 mg/ml $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular SPP1 / Osteopontin Antibodies

Anti-SPP1 / Osteopontin Antibody (N-Terminus) IHC-plus™ LS-B425
Rabbit Polyclonal to Human SPP1 / Osteopontin
Human, Mouse, Rat, Dog, Pig
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-SPP1 / Osteopontin Antibody (aa288-299) IHC-plus™ LS-B2411
Goat Polyclonal to Human SPP1 / Osteopontin
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human SPP1 / Osteopontin
Human, Guinea pig, Horse, Rabbit
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human SPP1 / Osteopontin
Rabbit
Human, Guinea pig, Horse, Rabbit (tested or 100% immunogen sequence identity)
Monkey, Dog (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with FITC, Biotin, HRP.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

SPP1 / Osteopontin antibody was raised against synthetic peptide from N-Terminus of human SPP1 (B2RDA1, NP_000573). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Monkey, Elephant, Rabbit, Horse (100%); Gibbon, Marmoset, Panda, Dog (92%); Guinea pig (85%); Platypus (84%).
LS-E13158 - Lyophilized - 100 µg - $145.00
N-Terminus
Human SPP1 / Osteopontin
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Packaging

Lyophilized from PBS with 2% sucrose
50 µl Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About SPP1 / Osteopontin

P10451 NM_000582 NP_000573.1

SPP1 Antibody, BSPI Antibody, BNSP Antibody, Bone sialoprotein 1 Antibody, ETA-1 Antibody, Nephropontin Antibody, OPN Antibody, Secreted phosphoprotein 1 Antibody, SPP-1 Antibody, SPP1/CALPHA1 fusion Antibody, Urinary stone protein Antibody, Uropontin Antibody, Osteopontin Antibody, Bone sialoprotein I Antibody

Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.

Immunohistochemistry

Immunohistochemistry
Anti-Osteopontin antibody IHC of human vessels. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5626 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
SPP1 / Osteopontin antibody LS-B5626 Western blot of THP-1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 12/2/2016

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