LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us
SPP1 / Osteopontin Antibody (Leu285) LS‑C176152
Osteopontin antibody LS-C176152 is an unconjugated rabbit polyclonal antibody to Osteopontin (SPP1) from human, mouse and rat. Validated for IHC and WB.
Catalog
Size
Price
LS-C176152-100
100 µl
Unavailable

Popular SPP1 / Osteopontin Products

SPP1 / Osteopontin antibody LS-C31763 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat, Bovine, Dog, Guinea pig, Pig
Applications: IHC, IHC - Paraffin, Western blot
Rabbit anti-Osteopontin was used at a 1:100-1:300 dilution to detect osteopontin by immunohistochemistry. Osteopontin is a normal component of elastic fibers in the breast (shown heavily stained in this section of human breast tumor cells). There is also weak staining of the extracellular matrix. Osteopontin is not expressed in breast tumor cells, and there is no staining of the breast cells in this section. No antigen retrieval is required.
Species: Human, Mouse, Rat, Dog, Pig
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Anti-Osteopontin antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2411 concentration 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Anti-Hemopexin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8326 dilution 1:50.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Rat, Rabbit
Applications: IHC, IHC - Paraffin, ICC, Western blot, Immunoprecipitation
Western blot of SPP1 / Osteopontin antibody LS-X223134.
Species: Human
Applications: Western blot, ELISA

Product Description

Osteopontin antibody LS-C176152 is an unconjugated rabbit polyclonal antibody to Osteopontin (SPP1) from human, mouse and rat. Validated for IHC and WB.
About SPP1 / Osteopontin
Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity. P10451 NM_000582 NP_000573.1

SPP1 Antibody, BSPI Antibody, BNSP Antibody, Bone sialoprotein 1 Antibody, ETA-1 Antibody, Nephropontin Antibody, OPN Antibody, Secreted phosphoprotein 1 Antibody, SPP-1 Antibody, SPP1/CALPHA1 fusion Antibody, Urinary stone protein Antibody, Uropontin Antibody, Osteopontin Antibody, Bone sialoprotein I Antibody


Specifications

Target
Human SPP1 / Osteopontin
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide around Leu285 of human Osteopontin
Epitope
Leu285
Specificity
Human Osteopontin
Presentation
PBS, pH 7.2, 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

Publications (0)


Customer Reviews (0)


Images


Western blot

Western blot of Osteopontin/SPP1 (L285) pAb in extracts from LOVO cells.
Western blot of Osteopontin/SPP1 (L285) pAb in extracts from LOVO cells.

Western blot

Western blot of Osteopontin/SPP1 (L285) pAb in extracts from LOVO cells.
Western blot of Osteopontin/SPP1 (L285) pAb in extracts from LOVO cells.

Western blot

Western blot of Osteopontin/SPP1 (L285) pAb in extracts from LOVO cells.
Western blot of Osteopontin/SPP1 (L285) pAb in extracts from LOVO cells.

Western blot

Western blot of Osteopontin/SPP1 (L285) pAb in extracts from LOVO cells.
Western blot of Osteopontin/SPP1 (L285) pAb in extracts from LOVO cells.


Requested From: 
Date Requested: 6/21/2018

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy



Catalog Number