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Anti-SPP1 / Osteopontin Antibody (clone 1B20, Biotin) LS-C180345

Catalog Size Price
LS-C180345-100 100 µl (0.8 mg/ml) Unavailable

Most Popular SPP1 / Osteopontin Antibodies

Anti-SPP1 / Osteopontin Antibody (C-Terminus) LS-C31763
Rabbit Polyclonal (IgG) to Human SPP1 / Osteopontin
Human, Mouse, Rat, Bovine, Dog, Guinea pig, Pig
IHC, IHC - Paraffin, Western blot
Unconjugated
SPP1 / Osteopontin antibody LS-C31763 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-SPP1 / Osteopontin Antibody (N-Terminus) IHC-plus™ LS-B425
Rabbit Polyclonal to Human SPP1 / Osteopontin
Human, Mouse, Rat, Dog, Pig
IHC, IHC - Paraffin, Western blot, ELISA
Unconjugated
Rabbit anti-Osteopontin was used at a 1:100-1:300 dilution to detect osteopontin by immunohistochemistry. Osteopontin is a normal component of elastic fibers in the breast (shown heavily stained in this section of human breast tumor cells). There is also weak staining of the extracellular matrix. Osteopontin is not expressed in breast tumor cells, and there is no staining of the breast cells in this section. No antigen retrieval is required.
Anti-SPP1 / Osteopontin Antibody (aa288-299) IHC-plus™ LS-B2411
Goat Polyclonal to Human SPP1 / Osteopontin
Human
IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Unconjugated
Anti-Osteopontin antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2411 concentration 5 ug/ml.
Anti-SPP1 / Osteopontin Antibody (C-Terminus, clone 1B20) IHC-plus™ LS-B8326
Mouse Monoclonal [clone 1B20] (IgG1) to Human SPP1 / Osteopontin
Human, Rat, Rabbit
IHC, IHC - Paraffin, ICC, Western blot, Immunoprecipitation
Unconjugated
Anti-Hemopexin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8326 dilution 1:50.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-SPP1 / Osteopontin Antibody (clone 2F10) LS-C305907
Mouse Monoclonal [clone 2F10] (IgG1,k) to Human SPP1 / Osteopontin
Human
Western blot, ELISA
Unconjugated
Western blot of SPP1 / Osteopontin antibody LS-X223134.

100% Guaranteed
Mouse Monoclonal [clone 1B20] (IgG1) to Human SPP1 / Osteopontin
Human, Rat, Rabbit
IHC, IHC - Paraffin, ICC, Western blot, Immunoprecipitation (applications tested for the base form of this product only)
Biotin Conjugated

Details

Human SPP1 / Osteopontin
Mouse
Human, Rat, Rabbit (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [1B20]
Biotin. Also available Unconjugated or conjugated with DY650, DY488, DY550.
Protein A purified
Unmodified

Applications

  • IHC
  • IHC - Paraffin (1:100)
  • ICC (1:50)
  • Western blot (1:1000)
  • Immunoprecipitation (1:10 - 1:500)
  • (applications tested for the base form of this product only)

Specificity and Use

A synthetic peptide corresponding to the C-terminus of human Osteopontin [Swiss-Prot# P10451].
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Packaging

PBS, 0.05% sodium azide
Store at 4°C in the dark.
For research use only.

About SPP1 / Osteopontin

P10451 NM_000582 NP_000573.1

SPP1 Antibody, BSPI Antibody, BNSP Antibody, Bone sialoprotein 1 Antibody, ETA-1 Antibody, Nephropontin Antibody, OPN Antibody, Secreted phosphoprotein 1 Antibody, SPP-1 Antibody, SPP1/CALPHA1 fusion Antibody, Urinary stone protein Antibody, Uropontin Antibody, Osteopontin Antibody, Bone sialoprotein I Antibody

Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.

Immunohistochemistry

Anti-Hemopexin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8326 dilution 1:50.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-Hemopexin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8326 dilution 1:50. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Osteopontin Antibody (1B20) - IHC of Osteopontin on human lung adenocarcinoma.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Osteopontin Antibody (1B20) - IHC of Osteopontin on human lung adenocarcinoma. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunocytochemistry

Osteopontin Antibody (1B20) - Osteopontin antibody was tested at 1:50 in U2OS cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Osteopontin Antibody (1B20) - Osteopontin antibody was tested at 1:50 in U2OS cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Osteopontin Antibody (1B20) - Western blot of Osteopontin expression in U2OS whole cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Osteopontin Antibody (1B20) - Western blot of Osteopontin expression in U2OS whole cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 5/21/2018

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