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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SPP1 / Osteopontin Antibody (C‑Terminus, clone 1B20) IHC‑plus™ LS‑B8326
Note: This antibody replaces LS-C142895
Osteopontin antibody LS-B8326 is an unconjugated mouse monoclonal antibody to Osteopontin (SPP1) from human, rat and rabbit. Validated for ICC, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B8326-100
100 µl (1 mg/ml)
Unavailable

Popular SPP1 / Osteopontin Products

SPP1 / Osteopontin antibody LS-C31763 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat, Bovine, Dog, Guinea pig, Pig
Applications: IHC, IHC - Paraffin, Western blot
Rabbit anti-Osteopontin was used at a 1:100-1:300 dilution to detect osteopontin by immunohistochemistry. Osteopontin is a normal component of elastic fibers in the breast (shown heavily stained in this section of human breast tumor cells). There is also weak staining of the extracellular matrix. Osteopontin is not expressed in breast tumor cells, and there is no staining of the breast cells in this section. No antigen retrieval is required.
Species: Human, Mouse, Rat, Dog, Pig
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Anti-Osteopontin antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2411 concentration 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Western blot of SPP1 / Osteopontin antibody LS-X223134.
Species: Human
Applications: Western blot, ELISA

Product Description

Osteopontin antibody LS-B8326 is an unconjugated mouse monoclonal antibody to Osteopontin (SPP1) from human, rat and rabbit. Validated for ICC, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
About SPP1 / Osteopontin
Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity. P10451 NM_000582 NP_000573.1

SPP1 Antibody, BSPI Antibody, BNSP Antibody, Bone sialoprotein 1 Antibody, ETA-1 Antibody, Nephropontin Antibody, OPN Antibody, Secreted phosphoprotein 1 Antibody, SPP-1 Antibody, SPP1/CALPHA1 fusion Antibody, Urinary stone protein Antibody, Uropontin Antibody, Osteopontin Antibody, Bone sialoprotein I Antibody


Specifications

Target
Human SPP1 / Osteopontin
Host
Mouse
Reactivity
Human, Rat, Rabbit (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [1B20]
Conjugations
Unconjugated. Also available conjugated with Biotin, DY650, DY488, DY550.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • ICC (1:50)
  • Western blot (1:1000)
  • Immunoprecipitation (1:1000)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide
Epitope
C-Terminus
Presentation
Tris-glycine, 150 mM sodium chloride, 0.05% sodium azide
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-Hemopexin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8326 dilution 1:50.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-Hemopexin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8326 dilution 1:50. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Osteopontin Antibody (1B20) - IHC of Osteopontin on human lung adenocarcinoma.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Osteopontin Antibody (1B20) - IHC of Osteopontin on human lung adenocarcinoma. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunocytochemistry

Osteopontin Antibody (1B20) - Osteopontin antibody was tested at 1:50 in U2OS cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Osteopontin Antibody (1B20) - Osteopontin antibody was tested at 1:50 in U2OS cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Osteopontin Antibody (1B20) - Western blot of Osteopontin expression in U2OS whole cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Osteopontin Antibody (1B20) - Western blot of Osteopontin expression in U2OS whole cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-Hemopexin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8326 dilution 1:50.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-Hemopexin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8326 dilution 1:50. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Osteopontin Antibody (1B20) - IHC of Osteopontin on human lung adenocarcinoma.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Osteopontin Antibody (1B20) - IHC of Osteopontin on human lung adenocarcinoma. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunocytochemistry

Osteopontin Antibody (1B20) - Osteopontin antibody was tested at 1:50 in U2OS cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Osteopontin Antibody (1B20) - Osteopontin antibody was tested at 1:50 in U2OS cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Osteopontin Antibody (1B20) - Western blot of Osteopontin expression in U2OS whole cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Osteopontin Antibody (1B20) - Western blot of Osteopontin expression in U2OS whole cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-Hemopexin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8326 dilution 1:50.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-Hemopexin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8326 dilution 1:50. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Osteopontin Antibody (1B20) - IHC of Osteopontin on human lung adenocarcinoma.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Osteopontin Antibody (1B20) - IHC of Osteopontin on human lung adenocarcinoma. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunocytochemistry

Osteopontin Antibody (1B20) - Osteopontin antibody was tested at 1:50 in U2OS cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Osteopontin Antibody (1B20) - Osteopontin antibody was tested at 1:50 in U2OS cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Osteopontin Antibody (1B20) - Western blot of Osteopontin expression in U2OS whole cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Osteopontin Antibody (1B20) - Western blot of Osteopontin expression in U2OS whole cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-Hemopexin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8326 dilution 1:50.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-Hemopexin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8326 dilution 1:50. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Osteopontin Antibody (1B20) - IHC of Osteopontin on human lung adenocarcinoma.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Osteopontin Antibody (1B20) - IHC of Osteopontin on human lung adenocarcinoma. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunocytochemistry

Osteopontin Antibody (1B20) - Osteopontin antibody was tested at 1:50 in U2OS cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Osteopontin Antibody (1B20) - Osteopontin antibody was tested at 1:50 in U2OS cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Osteopontin Antibody (1B20) - Western blot of Osteopontin expression in U2OS whole cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Osteopontin Antibody (1B20) - Western blot of Osteopontin expression in U2OS whole cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/21/2018

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