LSBio
Products
  • Antibodies
  • All Antibodies
  • Primary Antibodies
  • Secondary Antibodies
  • IHC-plus Antibodies
  • Isotype Control Antibodies
  • ELISA and Assay Kits
  • All Kits
  • Assay Kits
  • Traditional ELISA Kits
  • Cell-Based ELISA Kits
  • DNA-Binding ELISA Kits
  • Phospho-Specific ELISA Kits
  • ELISA Development Kits
  • Chemiluminescent CLIA Kits
  • Proteins
  • All Proteins
  • Recombinant Proteins
  • Native Proteins
  • Over-expression Lysates
  • Cell and Tissue Lysates
  • Bio-active Proteins
  • Animal-free Proteins
  • Synthetic Peptides
  • Biochemicals
  • All Biochemicals

  • Immunohistochemistry
  • Immunohistochemistry Reagents
  • Comprehensive IHC Reports
  • Custom IHC Services
  • TCR Screening Services
  • IHC-plus Antibodies
Services
  • Immunohistochemistry Services

  • Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

  • TCR Screening Services

  • Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Have a question? CustomerSupport@LSBio.com
Resources
  • Purchasing
  • Product Ordering Terms and Conditions
  • Holiday Schedule
  • About the LSBio Guarantee
  • About Reviews
  • Reference Material
  • About LSBio (LifeSpan BioSciences Inc.)
  • About Our 2-million Specimen Tissue Bank
  • About Our IHC Validation Process
  • About Our IHC-plus™ Immunohistochemistry Protocol
  • Protocols
  • Secure Logins
  • Login to Our Secure ShareFile Site
  • Login to Our IHC Database
Distributors
Contact Us

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)
How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Login ▾
Wish List
View Cart

Anti-SPP1 / Osteopontin Antibody (aa273-301) LS-C99283

Catalog Size Price
LS-C99283-400 400 µl Unavailable

Most Popular SPP1 / Osteopontin Antibodies

Anti-SPP1 / Osteopontin Antibody (C-Terminus) LS-C31763
Rabbit Polyclonal (IgG) to Human SPP1 / Osteopontin
Human, Mouse, Rat, Bovine, Dog, Guinea pig, Pig
IHC, IHC - Paraffin, Western blot
Unconjugated
SPP1 / Osteopontin antibody LS-C31763 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-SPP1 / Osteopontin Antibody (N-Terminus) IHC-plus™ LS-B425
Rabbit Polyclonal to Human SPP1 / Osteopontin
Human, Mouse, Rat, Dog, Pig
IHC, IHC - Paraffin, Western blot, ELISA
Unconjugated
Rabbit anti-Osteopontin was used at a 1:100-1:300 dilution to detect osteopontin by immunohistochemistry. Osteopontin is a normal component of elastic fibers in the breast (shown heavily stained in this section of human breast tumor cells). There is also weak staining of the extracellular matrix. Osteopontin is not expressed in breast tumor cells, and there is no staining of the breast cells in this section. No antigen retrieval is required.
Anti-SPP1 / Osteopontin Antibody (aa288-299) IHC-plus™ LS-B2411
Goat Polyclonal to Human SPP1 / Osteopontin
Human
IHC, IHC - Paraffin, Western blot, ELISA
Unconjugated
Anti-Osteopontin antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2411 concentration 5 ug/ml.
Anti-SPP1 / Osteopontin Antibody (C-Terminus, clone 1B20) IHC-plus™ LS-B8326
Mouse Monoclonal [clone 1B20] (IgG1) to Human SPP1 / Osteopontin
Human, Rat, Rabbit
IHC, IHC - Paraffin, ICC, Western blot, Immunoprecipitation
Unconjugated
Anti-Hemopexin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8326 dilution 1:50.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-SPP1 / Osteopontin Antibody (clone 2F10) LS-C305907
Mouse Monoclonal [clone 2F10] (IgG1,k) to Human SPP1 / Osteopontin
Human
Western blot, ELISA
Unconjugated
Western blot of SPP1 / Osteopontin antibody LS-X223134.

100% Guaranteed
Rabbit Polyclonal to Human SPP1 / Osteopontin
Human
IHC, IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated

Details

Human SPP1 / Osteopontin
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Ammonium sulfate precipitation
Unmodified

Applications

  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)

Specificity and Use

LS-E8284 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C99283. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa273-301
This SPP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 273-301 amino acids from the C-terminal region of human SPP1.

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About SPP1 / Osteopontin

P10451 NM_000582 NP_000573.1

SPP1 Antibody, BSPI Antibody, BNSP Antibody, Bone sialoprotein 1 Antibody, ETA-1 Antibody, Nephropontin Antibody, OPN Antibody, Secreted phosphoprotein 1 Antibody, SPP-1 Antibody, SPP1/CALPHA1 fusion Antibody, Urinary stone protein Antibody, Uropontin Antibody, Osteopontin Antibody, Bone sialoprotein I Antibody

Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human lung carcinoma with SPP1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human lung carcinoma with SPP1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot

Western blot of SPP1 Antibody in NCI-H460 cell line lysates (35 ug/lane). SPP1 (arrow) was detected using the purified antibody.
Western blot of SPP1 Antibody in NCI-H460 cell line lysates (35 ug/lane). SPP1 (arrow) was detected using the purified antibody.

Flow Cytometry

Flow cytometric of NCI-H292 cells using SPP1 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Flow cytometric of NCI-H292 cells using SPP1 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Requested From: 
Date Requested: 4/24/2018

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy



Catalog Number