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Anti-SPO11 Antibody (aa110-159) LS-C81959

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl - $365
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular SPO11 Antibodies

Anti-SPO11 Antibody (aa110-159) LS-C81959
Rabbit Polyclonal (IgG) to Human SPO11
Human, Monkey, Bat, Bovine, Dog, Guinea pig, Pig, Zebrafish
Western blot
Unconjugated
Western blot Image
Anti-SPO11 Antibody (aa299-326, AP) LS-C241195
Rabbit Polyclonal (IgG) to Human SPO11
Human
Western blot, ELISA
AP Conjugated
Anti-SPO11 Antibody (aa299-326, FITC) LS-C241198
Rabbit Polyclonal (IgG) to Human SPO11
Human
Western blot, ELISA
FITC Conjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human SPO11
Human, Monkey, Bat, Bovine, Dog, Guinea pig, Pig, Zebrafish
Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human SPO11
Rabbit
Human, Monkey, Bat, Bovine, Dog, Guinea pig, Pig, Zebrafish (tested or 100% immunogen sequence identity)
Mouse, Rat, Horse, Rabbit (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • Western blot
  • (applications tested for the base form of this product only)

Specificity and Use

SPO11 antibody was raised against synthetic peptide located between aa110-159 of human SPO11 (Q9Y5K1, NP_036576). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Galago, Marmoset, Elephant, Panda, Dog, Bovine, Bat, Pig, Opossum, Guinea pig (100%); Mouse, Rat, Rabbit, Horse, Zebra finch, Platypus (92%); Turkey, Chicken (84%); Stickleback (81%).
LS-E19799 - Lyophilized - 100 µg - $145.00
aa110-159
Human SPO11
ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Packaging

Lyophilized from PBS with 2% sucrose
50 µl Distilled Water.
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About SPO11

Q9Y5K1 NM_012444 NP_036576.1

SPO11 Antibody, Cancer/testis antigen 35 Antibody, CT35 Antibody

Meiotic recombination and chromosome segregation require the formation of double-strand breaks (DSBs) in paired chromosome homologs. During meiosis in yeast, a meiotic recombination protein is covalently-linked to the 5' end of DSBs and is essential for the formation of DSBs. The protein encoded by this gene is similar in sequence and conserved features to the yeast meiotic recombination protein. The encoded protein belongs to the TOP6A protein family.

Western blot

Western blot
HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 2/22/2017

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