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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SPN / CD43 Antibody (PE) LS‑C13414
CD43 antibody LS-C13414 is a PE-conjugated mouse monoclonal antibody to rat CD43 (SPN). Validated for Flow.
Antibody
Catalog
Size
Price
LS-C13414-100
100 tst
$620

Popular SPN / CD43 Products

Antibody
Species: Human
Applications: Western blot, ELISA
Anti-SPN / CD43 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3775 concentration 20 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry
Antibody
Species: Rat
Applications: Flow Cytometry
Staining of C57Bl/6 splenocytes with APC anti-mo/hu B220 (RA3-6B2) (LS-C107315) and.25 ug of Biotin anti-mouse CD43 antibody followed by SAv-PE. Cells in the lymphocyte gate were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Mouse
Applications: Flow Cytometry
Staining of C57Bl/6 splenocytes with.25 ug of Purified Rat IgM isotype control (open histogram) or.25 ug of Purified anti-mouse CD43 antibody (colored histogram) followed by PE anti-rat IgM (HIS40) (LS-C107152). Cells in the lymphocyte gate were used for analysis.
Species: Mouse
Applications: Western blot, Immunoprecipitation, Flow Cytometry

Product Description

CD43 antibody LS-C13414 is a PE-conjugated mouse monoclonal antibody to rat CD43 (SPN). Validated for Flow.
About SPN / CD43
One of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter-receptor for SN/Siglec-1 (By similarity). P16150 NM_003123 NP_003114.1

SPN Antibody, GPL115 Antibody, Leukosialin Antibody, Leukocyte sialoglycoprotein Antibody, Galactoglycoprotein Antibody, GALGP Antibody, Sialophorin Antibody, LSN Antibody, CD43 Antibody, CD43 antigen Antibody


Specifications

Target
Rat SPN / CD43
Host
Mouse
Reactivity
Rat (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Conjugations
Phycoerythrin
Purification
Protein G purified
Modifications
Unmodified
Applications
  • Flow Cytometry
Immunogen
SPN / CD43 antibody was raised against rat thymocyte membrane preparation.
Specificity
3H1324 is against a monomorphic determinant expressed on rat thymocytes, T lymphocytes (but not B lymphocytes), plasma cells, polymorphs, stem cells and brain. The antigen recognized is a heavily glycosylated glycoprotein of Mw 95000 which has a high content of O-linked carbohydrate structures. On thymocytes this glycoprotein is the main target for binding of peanut lectin. This antibody is useful for distinguishing B and T lymphocytes and also for analysis of NK cells and stem cells.
Usage
Flow Cytometry: Neat. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from PBS, pH 7.2, 0.1% Sodium Azide, 1% BSA
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: United States
Date Requested: 8/20/2018

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