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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SPN / CD43 Antibody (clone L11/135) LS‑C13407
CD43 antibody LS-C13407 is an unconjugated mouse monoclonal antibody to CD43 (SPN) from human and rabbit. Validated for Flow, IHC and IP.
Antibody
Catalog
Size
Price
LS-C13407-2000
2000 µl
$725

Popular SPN / CD43 Products

Antibody
Species: Rat
Applications: Flow Cytometry
Staining of C57Bl/6 splenocytes with.25 ug of Purified Rat IgM isotype control (open histogram) or.25 ug of Purified anti-mouse CD43 antibody (colored histogram) followed by PE anti-rat IgM (HIS40) (LS-C107152). Cells in the lymphocyte gate were used for analysis.
Species: Mouse
Applications: Western blot, Immunoprecipitation, Flow Cytometry
Staining of C57Bl/6 splenocytes with APC anti-mo/hu B220 (RA3-6B2) (LS-C107315) and.25 ug of Biotin anti-mouse CD43 antibody followed by SAv-PE. Cells in the lymphocyte gate were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Mouse
Applications: Flow Cytometry
Anti-SPN / CD43 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3775 concentration 20 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry
Antibody
Species: Human
Applications: Western blot, ELISA

Product Description

CD43 antibody LS-C13407 is an unconjugated mouse monoclonal antibody to CD43 (SPN) from human and rabbit. Validated for Flow, IHC and IP.
About SPN / CD43
One of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter-receptor for SN/Siglec-1 (By similarity). P16150 NM_003123 NP_003114.1

SPN Antibody, GPL115 Antibody, Leukosialin Antibody, Leukocyte sialoglycoprotein Antibody, Galactoglycoprotein Antibody, GALGP Antibody, Sialophorin Antibody, LSN Antibody, CD43 Antibody, CD43 antigen Antibody


Specifications

Target
Rabbit SPN / CD43
Host
Mouse
Reactivity
Rabbit, Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [L11/135]
Conjugations
Unconjugated
Purification
Tissue culture supernatant
Modifications
Unmodified
Applications
  • IHC
  • IHC - Frozen
  • Immunoprecipitation
  • Flow Cytometry
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
SPN / CD43 antibody was raised against glycoproteins from the RL-5 T-cell line.
Specificity
Recognizes rabbit CD34. Recognizes all T cells and T-Cell dependent areas in lymphoid tissue sections. Monocytes and macrophages stain weakly. Immunoprecipitates and immunoblots a 120kD protein from RL-5 cells and T lymphocytes. It is believed to recognize the homologue of Human CD43, Leukosialin.
Usage
Suitable for use in Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Flow Cytometry: Use 20 ul to label 100 ul whole blood or 1E6 cells in 100ul. Immunohistochemistry: Frozen sections.
Presentation
0.2 M Tris-HCl, pH 7.4, 0.09% Sodium Azide, 5%-10% FCS
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: United States
Date Requested: 8/16/2018

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