CD43 antibody LS-C13407 is an unconjugated mouse monoclonal antibody to CD43 (SPN) from human and rabbit. Validated for Flow, IHC and IP.
(tested or 100% immunogen sequence identity)
Tissue culture supernatant
- IHC - Frozen
- Flow Cytometry
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SPN / CD43 antibody was raised against glycoproteins from the RL-5 T-cell line.
Recognizes rabbit CD34. Recognizes all T cells and T-Cell dependent areas in lymphoid tissue sections. Monocytes and macrophages stain weakly. Immunoprecipitates and immunoblots a 120kD protein from RL-5 cells and T lymphocytes. It is believed to recognize the homologue of Human CD43, Leukosialin.
Suitable for use in Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Flow Cytometry: Use 20 ul to label 100 ul whole blood or 1E6 cells in 100ul. Immunohistochemistry: Frozen sections.
0.2 M Tris-HCl, pH 7.4, 0.09% Sodium Azide, 5%-10% FCS
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.