Monoclonal Mouse anti‑Human SPN / CD43 Antibody (clone CDLA43, Azide‑free,BSA‑free, IHC, IF, WB) LS‑C392078
Monoclonal Mouse anti‑Human SPN / CD43 Antibody (clone CDLA43, Azide‑free,BSA‑free, IHC, IF, WB) LS‑C392078
Antibody:
SPN / CD43 Mouse anti-Human Monoclonal (Azide-free, BSA-free) (CDLA43) Antibody
Application:
IHC, IHC-P, IF, WB, Flo
Format:
Unconjugated, Azide-free, BSA-free
Overview
Antibody:
SPN / CD43 Mouse anti-Human Monoclonal (Azide-free, BSA-free) (CDLA43) Antibody
Application:
IHC, IHC-P, IF, WB, Flo
Format:
Unconjugated, Azide-free, BSA-free
Specifications
Description
CD43 antibody LS-C392078 is an unconjugated mouse monoclonal antibody to human CD43 (SPN). Validated for Flow, IF, IHC and WB.
Target
Human SPN / CD43
Synonyms
SPN | GPL115 | Leukosialin | Leukocyte sialoglycoprotein | Galactoglycoprotein | GALGP | Sialophorin | LSN | CD43 | CD43 antigen
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k
Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Azide-free, BSA-free.
Also available
Unmodified or
Ready-to-Use.
Immunogen
Recombinant Full-length Human Protein.
Specificity
Human SPN / CD43
Applications
- IHC
- IHC - Paraffin (1 - 2 µg/ml)
- Immunofluorescence (1 - 2 µg/ml)
- Western blot (1 - 2 µg/ml)
- Flow Cytometry (0.5 - 1 µg/10E6 cells)
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Usage
The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. CD43 is one of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter-receptor for SN/Siglec-1 (By similarity). During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response (By similarity). [UniProt] Titering of the CD43 antibody may be required for optimal performance. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
Storage
Store at 2°C to 8°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
Featured Products
Species:
Rat
Applications:
Flow Cytometry
Species:
Human, Mouse
Applications:
IHC, Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, Immunoprecipitation, Flow Cytometry
Species:
Human
Applications:
IHC, Western blot, ELISA
Species:
Mouse
Applications:
IHC, Western blot, Immunoprecipitation, Flow Cytometry
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, Immunoprecipitation, Flow Cytometry
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 3/29/2024