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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SPN / CD43 Antibody (clone 3G8) LS‑C198254
CD43 antibody LS-C198254 is an unconjugated mouse monoclonal antibody to human CD43 (SPN). Validated for ELISA and WB.
Catalog
Size
Price
LS-C198254-100
100 µg
Unavailable

Popular SPN / CD43 Products

Antibody
Species: Rat
Applications: Flow Cytometry
Staining of C57Bl/6 splenocytes with.25 ug of Purified Rat IgM isotype control (open histogram) or.25 ug of Purified anti-mouse CD43 antibody (colored histogram) followed by PE anti-rat IgM (HIS40) (LS-C107152). Cells in the lymphocyte gate were used for analysis.
Species: Mouse
Applications: Western blot, Immunoprecipitation, Flow Cytometry
Staining of C57Bl/6 splenocytes with APC anti-mo/hu B220 (RA3-6B2) (LS-C107315) and.25 ug of Biotin anti-mouse CD43 antibody followed by SAv-PE. Cells in the lymphocyte gate were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Mouse
Applications: Flow Cytometry
Anti-SPN / CD43 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3775 concentration 20 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry
Antibody
Species: Human
Applications: Western blot, ELISA

Product Description

CD43 antibody LS-C198254 is an unconjugated mouse monoclonal antibody to human CD43 (SPN). Validated for ELISA and WB.
About SPN / CD43
One of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter-receptor for SN/Siglec-1 (By similarity). P16150 NM_003123 NP_003114.1

SPN Antibody, GPL115 Antibody, Leukosialin Antibody, Leukocyte sialoglycoprotein Antibody, Galactoglycoprotein Antibody, GALGP Antibody, Sialophorin Antibody, LSN Antibody, CD43 Antibody, CD43 antigen Antibody


Specifications

Target
Human SPN / CD43
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal [3G8]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • Western blot
  • ELISA
Immunogen
SPN / CD43 antibody was raised against sPN (AAH12350, aa1-400 full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human CD43
Usage
Sandwich ELISA: Recombinant protein. Western Blot: Transfected lysate, Recombinant protein.
Presentation
PBS, pH 7.4
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Western Blot analysis of SPN expression in transfected 293T cell line by SPN monoclonal antibody (M01), clone 3G8.Lane 1: SPN transfected lysate(59.327 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of SPN expression in transfected 293T cell line by SPN monoclonal antibody (M01), clone 3G8.Lane 1: SPN transfected lysate(59.327 KDa).Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged SPN is 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged SPN is 0.03 ng/ml as a capture antibody.

Western blot

Western Blot analysis of SPN expression in transfected 293T cell line by SPN monoclonal antibody (M01), clone 3G8.Lane 1: SPN transfected lysate(59.327 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of SPN expression in transfected 293T cell line by SPN monoclonal antibody (M01), clone 3G8.Lane 1: SPN transfected lysate(59.327 KDa).Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged SPN is 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged SPN is 0.03 ng/ml as a capture antibody.

Western blot

Western Blot analysis of SPN expression in transfected 293T cell line by SPN monoclonal antibody (M01), clone 3G8.Lane 1: SPN transfected lysate(59.327 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of SPN expression in transfected 293T cell line by SPN monoclonal antibody (M01), clone 3G8.Lane 1: SPN transfected lysate(59.327 KDa).Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged SPN is 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged SPN is 0.03 ng/ml as a capture antibody.

Western blot

Western Blot analysis of SPN expression in transfected 293T cell line by SPN monoclonal antibody (M01), clone 3G8.Lane 1: SPN transfected lysate(59.327 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of SPN expression in transfected 293T cell line by SPN monoclonal antibody (M01), clone 3G8.Lane 1: SPN transfected lysate(59.327 KDa).Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged SPN is 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged SPN is 0.03 ng/ml as a capture antibody.


Requested From: 
Date Requested: 6/21/2018

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