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| Catalog Number | Size | Price |
|---|---|---|
| LS-C17272-200 | 200 µg | $786 |
Monoclonal Mouse anti‑Human sPLA2 Antibody (WB) LS‑C17272
Monoclonal Mouse anti‑Human sPLA2 Antibody (WB) LS‑C17272
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Specifications
Description
SPLA2 antibody LS-C17272 is an unconjugated mouse monoclonal antibody to human sPLA2. Validated for ELISA and WB.
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k
Monoclonal
Purification
Protein G purified
Modifications
Unmodified
Immunogen
HPLC-purified human sperm phospholipase A2 (PLA2).
Specificity
Recognizes human type II secretory PLA2 at 14kD. Recognizes PLA2 in sperm, disc and synovial fluid.
Applications
- Western blot (5 - 10 µg/ml)
- ELISA (1:300)
Usage
Suitable for use in ELISA and Western Blot. Western Blot: 5-10 ug/ml detects 10 ng PLA2 under non-reducing conditions. Human synovial PLA2 was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-PLA2, secretory (5 ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. ELISA: 1:300 detects 3-28 ng PLA2.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Publications (0)
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Featured Products
Reactivity:
Porcine
Range:
0.31-20 ng/ml
Species:
Mouse, Rat
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Reactivity:
Human
Range:
0.156-10 ng/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/23/2024
Date Requested: 4/23/2024
