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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SPI1 / PU.1 Antibody (C‑Terminus) LS‑C203294
PU.1 antibody LS-C203294 is an unconjugated rabbit polyclonal antibody to human PU.1 (SPI1). Validated for WB.
Catalog
Size
Price
LS-C203294-40
40 µg (0.5 mg/ml)
Unavailable

Popular SPI1 / PU.1 Products

Antibody
Species: Human, Monkey, Mouse, Pig
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry, ELISA, Chromatin Immunoprecipitation
Anti-SPI1 / PU.1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3822 concentration 3.75 ug/ml.
Species: Human, Bovine, Dog, Horse, Pig, Rabbit
Applications: IHC, IHC - Paraffin
Anti-SPI1 / PU.1 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4206 concentration 10 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
 This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Mouse, Human
Applications: Western blot
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA

Product Description

PU.1 antibody LS-C203294 is an unconjugated rabbit polyclonal antibody to human PU.1 (SPI1). Validated for WB.
About SPI1 / PU.1
Binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing. P17947 NM_003120 NP_003111.2

SPI1 Antibody, 31 kDa-transforming protein Antibody, 31 kDa transforming protein Antibody, PU.1 Antibody, SFPI1 Antibody, SPI-1 Antibody, SPI-1 proto-oncogene Antibody, SPI-A Antibody, Transcription factor PU.1 Antibody


Specifications

Target
Human SPI1 / PU.1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1 - 2 µg/ml)
Immunogen
SPI1 / PU.1 antibody was raised against kLH-coupled synthetic peptide from C-terminal of human PU.1 (Swiss Prot: P17947)
Epitope
C-Terminus
Specificity
Human PU.1 protein.
Presentation
Lyophilized from PBS, pH 7.4, 0.02% sodium azide.
Reconstitution
80 µl Distilled water
Storage
Store lyophilized at -20°C. The reconstituted product can be stored for short term at 4°C or long term at -20°C or below. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Western blot analysis of extracts from HEK293 cells transfected with human PU.1 for 72hr using PU.1 Antibody, pAb, Rabbit The signal was developed with IRDye TM 800 Conjugated Goat Anti-Rabbit IgG. Predicted Size: 31 kD Observed Size: 31 kD
Western blot analysis of extracts from HEK293 cells transfected with human PU.1 for 72hr using PU.1 Antibody, pAb, Rabbit The signal was developed with IRDye TM 800 Conjugated Goat Anti-Rabbit IgG. Predicted Size: 31 kD Observed Size: 31 kD

Western blot

Western blot analysis of human recombinant PU.1 fusion protein using PU.1 Antibody, pAb, Rabbit The signal was developed with IRDye TM 800 Conjugated Goat Anti-Rabbit IgG. Predicted Size: 62 kD Observed Size: 62 kD
Western blot analysis of human recombinant PU.1 fusion protein using PU.1 Antibody, pAb, Rabbit The signal was developed with IRDye TM 800 Conjugated Goat Anti-Rabbit IgG. Predicted Size: 62 kD Observed Size: 62 kD

Western blot

Western blot analysis of extracts from HEK293 cells transfected with human PU.1 for 72hr using PU.1 Antibody, pAb, Rabbit The signal was developed with IRDye TM 800 Conjugated Goat Anti-Rabbit IgG. Predicted Size: 31 kD Observed Size: 31 kD
Western blot analysis of extracts from HEK293 cells transfected with human PU.1 for 72hr using PU.1 Antibody, pAb, Rabbit The signal was developed with IRDye TM 800 Conjugated Goat Anti-Rabbit IgG. Predicted Size: 31 kD Observed Size: 31 kD

Western blot

Western blot analysis of human recombinant PU.1 fusion protein using PU.1 Antibody, pAb, Rabbit The signal was developed with IRDye TM 800 Conjugated Goat Anti-Rabbit IgG. Predicted Size: 62 kD Observed Size: 62 kD
Western blot analysis of human recombinant PU.1 fusion protein using PU.1 Antibody, pAb, Rabbit The signal was developed with IRDye TM 800 Conjugated Goat Anti-Rabbit IgG. Predicted Size: 62 kD Observed Size: 62 kD

Western blot

Western blot analysis of extracts from HEK293 cells transfected with human PU.1 for 72hr using PU.1 Antibody, pAb, Rabbit The signal was developed with IRDye TM 800 Conjugated Goat Anti-Rabbit IgG. Predicted Size: 31 kD Observed Size: 31 kD
Western blot analysis of extracts from HEK293 cells transfected with human PU.1 for 72hr using PU.1 Antibody, pAb, Rabbit The signal was developed with IRDye TM 800 Conjugated Goat Anti-Rabbit IgG. Predicted Size: 31 kD Observed Size: 31 kD

Western blot

Western blot analysis of human recombinant PU.1 fusion protein using PU.1 Antibody, pAb, Rabbit The signal was developed with IRDye TM 800 Conjugated Goat Anti-Rabbit IgG. Predicted Size: 62 kD Observed Size: 62 kD
Western blot analysis of human recombinant PU.1 fusion protein using PU.1 Antibody, pAb, Rabbit The signal was developed with IRDye TM 800 Conjugated Goat Anti-Rabbit IgG. Predicted Size: 62 kD Observed Size: 62 kD

Western blot

Western blot analysis of extracts from HEK293 cells transfected with human PU.1 for 72hr using PU.1 Antibody, pAb, Rabbit The signal was developed with IRDye TM 800 Conjugated Goat Anti-Rabbit IgG. Predicted Size: 31 kD Observed Size: 31 kD
Western blot analysis of extracts from HEK293 cells transfected with human PU.1 for 72hr using PU.1 Antibody, pAb, Rabbit The signal was developed with IRDye TM 800 Conjugated Goat Anti-Rabbit IgG. Predicted Size: 31 kD Observed Size: 31 kD

Western blot

Western blot analysis of human recombinant PU.1 fusion protein using PU.1 Antibody, pAb, Rabbit The signal was developed with IRDye TM 800 Conjugated Goat Anti-Rabbit IgG. Predicted Size: 62 kD Observed Size: 62 kD
Western blot analysis of human recombinant PU.1 fusion protein using PU.1 Antibody, pAb, Rabbit The signal was developed with IRDye TM 800 Conjugated Goat Anti-Rabbit IgG. Predicted Size: 62 kD Observed Size: 62 kD


Requested From: 
Date Requested: 6/21/2018

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