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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SPHK2 Antibody (aa580‑629) LS‑C118372
SPHK2 antibody LS-C118372 is an unconjugated rabbit polyclonal antibody to SPHK2 from human, mouse and rat. Validated for IHC, Peptide-ELISA and WB.
Catalog
Size
Price
LS-C118372-50
50 µl
Unavailable
LS-C118372-100
100 µl
Unavailable

Popular SPHK2 Products

Western blot of Sphingosine Kinase 2 antibody on 50 ng of recombinant Sphinogsine Kinase 2 enzyme. Antibody used at 1:200 dilution. Visualized using Pierce West Femto substrate system. Secondary used at 1:30k dilution. Exposure for 45 seconds.
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Applications: Western blot
Anti-SPHK2 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1101 concentration 5 ug/ml.
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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Western blot of SPHK2 antibody LS-C147009.
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Applications: Western blot

Product Description

SPHK2 antibody LS-C118372 is an unconjugated rabbit polyclonal antibody to SPHK2 from human, mouse and rat. Validated for IHC, Peptide-ELISA and WB.
About SPHK2
Unlike the proliferative action of SPHK1, SPHK2 has been shown to possess anti-proliferative or pro-apoptotic action. SphK2 suppresses growth and enhances apoptosis, independent of activation of S1P receptors. Sequence analysis revealed that SphK2 contains a 9 amino acid motif similar to that present in Bcl2-homology domain 3 (BH3)- only proteins, a pro-apoptotic subgroup of the Bcl-2 family. Q9NRA0 NM_020126 NP_064511.2

SPHK2 Antibody, SK-2 Antibody, Sk2 Antibody, Sphingosine kinase type 2 Antibody, SPK-2 Antibody, Sphingosine kinase 2 Antibody, SPHK2L Antibody, SK 2 Antibody, SPK 2 Antibody


Specifications

Target
Human SPHK2
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • Western blot (1:500 - 1:1000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:5000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
SPHK2 antibody was raised against synthetic peptide from human SPHK2 (aa580-629).
Epitope
aa580-629
Specificity
SPHK2 (Ab-614) Antibody detects endogenous levels of total SPHK2 protein.
Presentation
PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
Storage
Store at -20°C for up to 1 year.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

IHC of paraffin-embedded human breast carcinoma tissue, using SPHK2 (Ab-614) Antibody. The picture on the right is treated with the synthesized peptide.
IHC of paraffin-embedded human breast carcinoma tissue, using SPHK2 (Ab-614) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot

Western blot of extracts from 293 cells, using SPHK2 (Ab-614) Antibody. The lane on the right is treated with the synthesized peptide.
Western blot of extracts from 293 cells, using SPHK2 (Ab-614) Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry

IHC of paraffin-embedded human breast carcinoma tissue, using SPHK2 (Ab-614) Antibody. The picture on the right is treated with the synthesized peptide.
IHC of paraffin-embedded human breast carcinoma tissue, using SPHK2 (Ab-614) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot

Western blot of extracts from 293 cells, using SPHK2 (Ab-614) Antibody. The lane on the right is treated with the synthesized peptide.
Western blot of extracts from 293 cells, using SPHK2 (Ab-614) Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry

IHC of paraffin-embedded human breast carcinoma tissue, using SPHK2 (Ab-614) Antibody. The picture on the right is treated with the synthesized peptide.
IHC of paraffin-embedded human breast carcinoma tissue, using SPHK2 (Ab-614) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot

Western blot of extracts from 293 cells, using SPHK2 (Ab-614) Antibody. The lane on the right is treated with the synthesized peptide.
Western blot of extracts from 293 cells, using SPHK2 (Ab-614) Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry

IHC of paraffin-embedded human breast carcinoma tissue, using SPHK2 (Ab-614) Antibody. The picture on the right is treated with the synthesized peptide.
IHC of paraffin-embedded human breast carcinoma tissue, using SPHK2 (Ab-614) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot

Western blot of extracts from 293 cells, using SPHK2 (Ab-614) Antibody. The lane on the right is treated with the synthesized peptide.
Western blot of extracts from 293 cells, using SPHK2 (Ab-614) Antibody. The lane on the right is treated with the synthesized peptide.


Requested From: 
Date Requested: 6/25/2018

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