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Anti-SPHK / SPHK1 Antibody (aa59-89) LS-C100770

Catalog Size Price
LS-C100770-400 400 µl Unavailable

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100% Guaranteed
Rabbit Polyclonal to Human SPHK / SPHK1
Human
IHC - Paraffin, Western blot
Unconjugated

Details

Human SPHK / SPHK1
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Ammonium sulfate precipitation
Unmodified

Applications

  • IHC - Paraffin
  • Western blot (1:1000)

Specificity and Use

LS-E8788 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C100770. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa59-89
This SPHK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 59-89 amino acids from the N-terminal region of human SPHK1.

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About SPHK / SPHK1

Q9NYA1 NM_021972 NP_068807.2

SPHK1 Antibody, SK1 Antibody, Sphingosine kinase 1 Antibody, SPHK Antibody, SK 1 Antibody, SPK 1 Antibody, Sphingosine kinase type 1 Antibody, SPK Antibody

SPHK / SPHK1 catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G protein-coupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes.

Immunohistochemistry

Immunohistochemistry
Immunohistochemical of paraffin-embedded M. kidney section using SPHK1 Antibody(N-term P74). Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemistry

Immunohistochemistry
Immunohistochemical of paraffin-embedded H. kidney section using SPHK1 Antibody(N-term P74). Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Western blot

Western blot
Western blot of lysates from HepG2 cell line and human heart, skeletal muscle, kidney tissue lysate(from left to right), using SPHK1 Antibody (N-term P74). Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

Requested From: 
Date Requested: 6/23/2017

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