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Anti-SPHK / SPHK1 Antibody (aa334-384) LS-C142727

Ordering

Wt. Vol. Conc. Price
- 100 µl - $335
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular SPHK / SPHK1 Antibodies

Anti-SPHK / SPHK1 Antibody (N-Terminus) IHC-plus™ LS-A3106
Rabbit Polyclonal to Human SPHK / SPHK1
Human, Monkey
IHC - Paraffin
Unconjugated
Immunohistochemistry Image
Anti-SPHK / SPHK1 Antibody (aa176-189) LS-C87246
Goat Polyclonal to Human SPHK / SPHK1
Human, Monkey, Mouse, Hamster
Western blot, ELISA
Unconjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human SPHK / SPHK1
Human
Western blot, Immunoprecipitation
Unconjugated

Details

Human SPHK / SPHK1
Rabbit
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • Western blot (1:2000 - 1:10000)
  • Immunoprecipitation

Specificity and Use

SPHK / SPHK1 antibody was raised against the epitope recognized by this antibody maps to a region between residue 334 and 384 of human sphingosine kinase 1 using the numbering given in entry NP_001136073.1 (GeneID 8877)
aa334-384
SPHK1.

Packaging

Tris-citrate/phosphate buffer, pH 7 to 8, 0.09% sodium azide
Store at 4°C. Do not freeze.
For research use only.

About SPHK / SPHK1

Q9NYA1 NM_021972 NP_068807.2

SPHK1 Antibody, SK1 Antibody, Sphingosine kinase 1 Antibody, SPHK Antibody, SK 1 Antibody, SPK 1 Antibody, Sphingosine kinase type 1 Antibody, SPK Antibody

SPHK / SPHK1 catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G protein-coupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes.

Western blot

Western blot
SPHK1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti- SPHK1 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 10 mcg/mg lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/4/2016

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