Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
SPHK / SPHK1 catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G protein-coupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes.
Western blot of anti-hSPHK1-M1 antibody in 293 cell line lysates transiently transfected with the SPHK1 gene (2 ug/lane). hSPHK1-M1(arrow) was detected using the purified antibody.
The anti-SphK1 antibody is used in Western blot (Lane 2) to detect c-myc-tagged SphK1 in transfected 293 cell lysate (a c-myc antibody is used as control in Lane 1). Data is kindly provided by Dr. J. Van Brocklyn from the Ohio State University (Columbus, OH).
Western blot of lysate from A431 cell line, using SPHK1 Antibody (M1). Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.