LSBio The Immunohistochemistry Antibody Company
Products
  • Antibodies
  • All Antibodies
  • Primary Antibodies
  • Secondary Antibodies
  • IHC-plus Antibodies
  • Isotype Control Antibodies
  • ELISA Kits
  • All Kits
  • Sandwich ELISA Kits
  • Competitive EIA Kits
  • Cell-Based ELISA Kits
  • DNA-Binding ELISA Kits
  • Direct ELISA Kits
  • Functional ELISA Kits
  • Phospho-Specific ELISA Kits
  • Custom ELISA Kits
  • Proteins
  • All Proteins
  • Recombinant Proteins
  • Native Proteins
  • Over-expression Lysates
  • Cell and Tissue Lysates
  • Bio-active Proteins
  • Animal-free Proteins
  • Synthetic Peptides
  • Immunohistochemistry
  • Comprehensive IHC Reports
  • Custom IHC Services
  • TCR Screening Services
  • IHC-plus Antibodies
  • Other
  • Blocking Peptides
Services
  • Immunohistochemistry Services

  • Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

  • TCR Screening Services

  • Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Have a question? CustomerSupport@LSBio.com
Resources
  • Purchasing
  • Product Ordering Terms and Conditions
  • Holiday Schedule
  • About the LSBio Guarantee
  • About Our Rewards Program
  • Reference Material
  • About LSBio (LifeSpan BioSciences Inc.)
  • About Our 2-million Specimen Tissue Bank
  • About Our IHC Validation Process
  • About Our IHC-plus™ Immunohistochemistry Protocol
  • Publications
  • Secure Logins
  • Login to Our Secure FTP Site
  • Login to Our IHC Database
Distributors
Contact Us

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)
How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Quick Order ▾
View Cart

Anti-SPARCL1 / Hevin Antibody (aa17-664) LS-C150077

Ordering

Wt. Vol. Conc. Price
100 µg 500 µl 0.2 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular SPARCL1 / Hevin Antibodies

Anti-SPARCL1 / Hevin Antibody (aa17-650, clone 348905) LS-C39200
Rat Monoclonal [clone 348905] (IgG2a) to Mouse SPARCL1 / Hevin
Mouse
Western blot, ELISA
Unconjugated
Anti-SPARCL1 / Hevin Antibody (aa17-664) LS-C150077
Goat Polyclonal (IgG) to Human SPARCL1 / Hevin
Human
IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image
Anti-SPARCL1 / Hevin Antibody (aa649-664) LS-C313206
Rabbit Polyclonal (IgG) to Human SPARCL1 / Hevin
Human, Monkey, Mouse, Rat, Bat, Guinea pig, Horse, Rabbit
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal (IgG) to Human SPARCL1 / Hevin
Human
IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated

Details

Human SPARCL1 / Hevin
Goat
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 - 15 µg/ml)
  • Western blot (1 µg/ml)
  • Flow Cytometry

Specificity and Use

SPARCL1 / Hevin antibody was raised against mouse myeloma cell line NS0-derived recombinant human SPARC-like 1/SPARCL1 Ile17-Phe664 Accession # Q8N4S1.
aa17-664
Detects human SPARCL1 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with.
Western Blot, 1 µg/ml. Immunohistochemistry, 5-15 µg/ml, Intracellular Staining by Flow Cytometry, 2.5 µg/106 cells.

Packaging

Lyophilized from 0.2 µm filtered PBS, pH 7.4, 5% trehalose
500 µl Sterile PBS
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About SPARCL1 / Hevin

Q14515 NM_004684 NP_004675.3

SPARCL1 Antibody, Hevin Antibody, PIG33 Antibody, SC1 Antibody, Mast9 Antibody, SPARC-like 1 (hevin) Antibody, SPARC-like 1 (mast9, hevin) Antibody, SPARC-like protein 1 Antibody, MAST 9 Antibody


Immunohistochemistry

Immunohistochemistry
SPARC-like 1/SPARCL1 in Human Brain. SPARC-like 1/SPARCL1 was detected in immersion fixed paraffin-embedded sections of human brain using Goat Anti-Human SPARC-like 1/SPARCL1 Antigen Affinity-purified Polyclonal Antibody at 15 ug/ml overnight at 4°C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic. Tissue was stained using Anti-Goat HRP-DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neurons.

Western blot

Western blot
Detection of Human SPARC-like 1/SPARCL1 by Western Blot. Western blot shows lysates of human lung tissue. PVDF membrane was probed with 1 ug/ml of Goat Anti-Human SPARC-like 1/ SPARCL1 Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for SPARC-like 1/SPARCL1 at approximately 130 kD (as indicated). This experiment was conducted under reducing conditions.

Flow Cytometry

Flow Cytometry
Detectionof SPARClike1/SPARCL1 in HL60 Human Cell Line by Flow Cytometry. HL60 human acute promyelocytic leukemia cell line was stained with Goat Anti-Human SPARC-like 1/SPARCL1 Antigen Affinity-purified Polyclonal Antibody or control antibody, followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
 

Requested From: United States
Date Requested: 12/4/2016

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn
Copyright © 2016 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy
Catalog Number