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Anti-SPANXC / SPANX-C Antibody IHC-plus™ LS-B484

Note: This antibody replaces LS-C19115


Wt. Vol. Conc. Price
50 µg 50 µl 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular SPANXC / SPANX-C Antibodies

Anti-SPANXC / SPANX-C Antibody (aa31-47) IHC-plus™ LS-B424
Rabbit Polyclonal to Human SPANXC / SPANX-C
IHC - Paraffin, Western blot, ELISA
Immunohistochemistry Image
Anti-SPANXC / SPANX-C Antibody IHC-plus™ LS-B484
Rabbit Polyclonal to Human SPANXC / SPANX-C
IHC - Paraffin, Western blot, ELISA
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human SPANXC / SPANX-C
IHC - Paraffin, Western blot, ELISA


Human (tested or 100% immunogen sequence identity)
Protein A purified


  • IHC - Paraffin (2.5 µg/ml)
  • Western blot (1:1000 - 1:5000)
  • ELISA (1:5000 - 1:20000)

Specificity and Use

SPANXC / SPANX-C antibody was raised against recombinant human SPANXC.
Full-length recombinant human SPANX-C protein.
Immunohistochemistry: LS-B484 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B484 was determined to be 2.5 ug/ml.


0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
For research use only.


Q9NY87 NM_022661 NP_073152.2

SPANXC Antibody, CTp11 Antibody, CT11.3 Antibody, SPANX family member C Antibody, SPAN-Xc protein Antibody, SPANX family, member C Antibody, SPANX-C Antibody, Cancer/testis antigen 11.3 Antibody

Temporally regulated transcription and translation of several testis-specific genes is required to initiate the series of molecular and morphological changes in the male germ cell lineage necessary for the formation of mature spermatozoa. This gene is a member of the SPANX family, which is located in a gene cluster on chromosome X. The SPANX genes encode differentially expressed testis-specific proteins that localize to various subcellular compartments.


Anti-SPANXC antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B484 concentration 5 ug/ml.

Western blot

Western blot
Anti-SPANX-C Antibody - Western Blot. Western blot of Protein A purified anti-SPANX-C antibody shows detection of a band at ~17 kD corresponding to SPANX-C present in a nuclear extract from VWM105 cells (left panel, arrowhead). VWM105 cells are derived from a human melanoma and are positive for SPANX proteins. Lane 2 shows reactivity with a purified recombinant SPANX-C fusion protein. The right panel shows similar reactivity with purified recombinant SPANX-B, SPANX-C and SPANX-N proteins. Proteins were separated by SDS-PAGE, transferred to nitrocellulose, and probed with the primary antibody diluted to 1:1000. IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX ( was used (left). IRDye is a trademark of LI-COR, Inc. Size estimation was made by comparison to prestained MW markers as indicated. Personal Communication. Vladimir Larionov, NIH, CCR, Bethesda, MD.

Requested From: United States
Date Requested: 10/23/2016

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