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Anti-SPANXC / SPANX-C Antibody (aa31-47) IHC-plus™ LS-B424

Note: This antibody replaces LS-C19001

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
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Most Popular SPANXC / SPANX-C Antibodies

Anti-SPANXC / SPANX-C Antibody (aa31-47) IHC-plus™ LS-B424
Rabbit Polyclonal to Human SPANXC / SPANX-C
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-SPANXC / SPANX-C Antibody IHC-plus™ LS-B484
Rabbit Polyclonal to Human SPANXC / SPANX-C
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human SPANXC / SPANX-C
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human SPANXC / SPANX-C
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:500 - 1:3000)
  • ELISA (1:10000 - 1:50000)

Specificity and Use

SPANXC / SPANX-C antibody was raised against synthetic peptide from human SPANXC.
aa31-47
Amino acids 31-47 of human SPANX-C protein.
Immunohistochemistry: LS-B424 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B424 was determined to be 5 ug/ml.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About SPANXC / SPANX-C

Q9NY87 NM_022661 NP_073152.2

SPANXC Antibody, CTp11 Antibody, CT11.3 Antibody, SPANX family member C Antibody, SPAN-Xc protein Antibody, SPANX family, member C Antibody, SPANX-C Antibody, Cancer/testis antigen 11.3 Antibody

Temporally regulated transcription and translation of several testis-specific genes is required to initiate the series of molecular and morphological changes in the male germ cell lineage necessary for the formation of mature spermatozoa. This gene is a member of the SPANX family, which is located in a gene cluster on chromosome X. The SPANX genes encode differentially expressed testis-specific proteins that localize to various subcellular compartments.

Immunohistochemistry

Immunohistochemistry
Anti-SPANXC antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B424 concentration 5 ug/ml.

Western blot

Western blot
Anti-SPANX (pan) Antibody - Western Blot. Western blot of affinity purified anti-SPANX (pan) antibody shows detection of a band at ~17 kD corresponding to SPANX-C present in a nuclear extract from VWM105 cells (left panel, arrowhead). VWM105 cells are derived from a human melanoma and are positive for SPANX proteins. Lane 2 shows reactivity with a purified recombinant SPANX-C fusion protein. The right panel shows similar reactivity with purified recombinant SPANX-B, SPANX-C and SPANX-N proteins. Proteins were separated by SDS-PAGE, transferred to nitrocellulose, and probed with the primary antibody diluted to 1:1000. IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX ( was used (left). IRDye is a trademark of LI-COR, Inc. Size estimation was made by comparison to prestained MW markers as indicated. Personal Communication. Vladimir Larionov, NIH, CCR, Bethesda, MD.

Requested From: United States
Date Requested: 12/5/2016

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